维药索索葡萄中齐墩果酸薄层鉴别方法研究

TLC Identification of Oleanolic Acid in the Uyghur Medicine Vitis vinifera L

目的以齐墩果酸为对照品,制定新疆维吾尔药材索索葡萄的质量标准之薄层鉴别方法。方法收集三个产地(吐鲁番,鄯善和哈密),10个批次的索索葡萄生药材,根据《中国药典》(2010版)附录ⅥB(薄层色谱法)中相关项下方法进行薄层鉴别。以齐墩果酸作为对照品,考察不同提取方法和不同提取溶剂对齐墩果酸薄层的影响;考察不同展开系统、显色剂、温度、湿度、点样量和不同厂家硅胶板对索索葡萄中齐墩果酸薄层鉴别的影响,从中选出维药索索葡萄中齐墩果酸薄层鉴别的最佳条件。结果采用丙酮为溶剂,超声提取30 min,吸取供试品溶液和对照品溶液各2μl点于青岛海洋化工有限公司高效硅胶板之上,在环己烷-丙酮-乙酸乙酯-甲酸(9∶2∶1∶0.2)系统中展开,喷以10%硫酸乙醇显色,供试品与齐墩果酸对照品在同样的位置显紫色的斑点,色谱斑点清晰,能有效提供索索葡萄的鉴别特征。结论实验建立的索索葡萄药材中齐墩果酸的薄层鉴别方法,操作简便,重现性好,专属性强,能够为其薄层鉴别提供实验资料和科学依据。

Objective To set up the TLC identification of Vitis vinifera.L（Chinese name ＂Suosuo＂ grape,traditional Uyghur medicine） with oleanolic acid as standard compound. MethodsAccording to the Chinese Pharmacopoeia（2010 Revision） Appendix Ⅵ B（TLC）,different extract methods and solvents,developing system,chromogenic agents,temperature,humidity,drawing amounts and thin layer boards were investigated for optimizing TLC identification of Vitis vinifera L. ResultsUsing ultrasonic extraction 30min with acetone,drawing 2 μl solution on efficient thin layer board（200 mm × 100 mm,Qingdao Ocean Chemical Co.,Ltd.）,developing system cyclohexane-acetone-ethyl acetate-formic acid（9∶2∶1∶0.2） was applied.After spaying with 10% sulfuric acid in ethanol,significant purple color appeared on the sample solution with the oleanolic acid at same position. Conclusion The established TLC identification of Vitis vinifera L.（oleanolic acid as the standard compound） is simple,repeatable and specific.It can improve and lay a foundation on the quality standardization of Vitis vinifera L.