高效液相色谱法检测蔗渣半纤维素水解物中的单糖

Determination of Monosaccharide in Bagasse Hemicellulose Hydrolysate by High Performance Liquid Chromatography

用高效液相色谱法测定甘蔗渣半纤维素水解物中的单糖含量。以 Hypersil NH2 柱为色谱分析柱 ,用示差折光检测器检测 ,流动相是乙腈∶水 (80∶ 2 0 )。在此色谱条件下 ,甘蔗渣半纤维素水解物分离成 :木糖、阿拉伯糖、果糖和葡萄糖。各单糖呈良好的线性关系。加样回收率的平均值为 97.3%～ 98.7% ,此方法简便、快速、准确。

A method for the determination of monosaccharides in hemicellulose hydrolysate from sugar cane bagasse by HPLC(high performance liquid chromatography) was developed.The operating conditions were Hypersil NH 2(150mm×4 6mm i.d) column; mobile phase of V(acetonitrile)∶V(water)=80∶20; flow rate of 1 0 ml/min; detected with differential refractometer. Under this condition, the results are satisfactory. The method is simple, quick and accurate. The average recovery was 97 3% to 98 7%.