蛇源变形杆菌的分离鉴定

Isolation and Identification of Proteus from Diseased Snakes

取广西南宁、河池和柳州等地 140条病死及濒死蛇的心血等作细菌培养。结果分离出细菌 2 32株 ;分类鉴定发现 79株为变形杆菌 ,占检出菌的 34.1% ;种的分类表明 ,6 3株为普通变形杆菌 ,16株为奇异变形杆菌 ;随机抽取其中 5株进行毒素测定 ,均发现有肠毒素产生 ;做动物试验可致小白鼠死亡 ;做回归试验 ,可见蛇血带菌。说明 ,此变形杆菌具有一定的毒力 ,是造成蛇感染的常见菌之一 ,为人兽共患病的传染源 ,不可忽视。

bacteria were isolated from the heart blood samples of 140 die of illness or dying snakes collected in Nanning,Hechi and Liuzhou of Guangxi; The results of identification and classification revealed that 79 bacterial isolates belonged to the genus of Proteus, among them P vulgaria was the dominant species with 63 isolates,and P mirabilis with 16 isolates. Toxin test with 5 randomly chosen isolates showed positive. Pathogenicity tests with mouce and snake resulted in death and bacteremia respectively.The results demonstrated that proteus as a conditioned pathogen is an important agent in zoonosis.