耐核苷类药物的乙型肝炎病毒P区逆转录酶基因序列分析的研究

Analysis of reverse transcriptase gene sequences in P region of nucleoside analogs-resistant hepatitis B virus

目的探讨慢性乙型肝炎患者体内乙型肝炎病毒(HBV)P区逆转录酶(RT)基因突变发生的频率和类型。方法采用聚合酶链反应(PCR)方法对38例HBVRT基因进行扩增后测序,分析rt80、rt84、rt85、rt169、rt173、rt180、rt181、rt184、rt194、rt202、rt204、rt207、rt213、rt214、rt215、rt229、rt233、rt236、rt237、rt238、rt250位点的变异情况。结果 HBVRT基因突变主要集中于rt204、rt238、rt180、rt229,突变类型以M204I、N/H238T、L180M、M204V为主。结论对HBVRT基因的序列分析有利于临床选择有效的抗病毒药物进行治疗。

Objective To investigate the mutation frequencies and types of reverse transcriptase（RT）gene in P region of hepatitis B virus（HBV）from patients with chronic hepatitis B.Methods RT gene of HBV from 38 patients were amplified by polymerase chain reaction（PCR）and then sequenced.Point mutations of rt80,rt84,rt85,rt169,rt173,rt180 rt181,rt184,rt194,rt202,rt204,rt207,rt213,rt214,rt215,rt229,rt233,rt236,rt237,rt238,rt250 were analyzed.Results HBV RT gene mutations were mainly located on rt204,rt238,rt180,rt229.M204I,N/H238T,L180M,M204V were the major mutation types.Conclusion Sequence analysis of HBV RT gene is contribute to selecting effective antiviral drugs for clinical treatment.