MET4和C-28抗体检测卵巢上皮性肿瘤组织中MET蛋白表达的对比研究

A comparative study on MET protein expression in ovarian epithelial tumor tissue by MET4 and C-28 antibody detection

目的评价MET4和C-28抗体检测卵巢上皮性肿瘤组织中MET蛋白表达的效能。方法采用免疫组织化学间接法检测卵巢上皮性肿瘤、卵巢转移性腺癌和正常卵巢组织芯片中MET蛋白表达的情况,采用Nuance光谱成像分析系统对组织芯片的免疫染色进行分析。结果 MET4和C-28蛋白阳性染色部位均位于细胞膜和细胞质。MET4蛋白的阳性总检出率为64.2%(111/173),高于C-28[50.2%(87/173)](P<0.05),且MET4蛋白在卵巢原发性上皮性肿瘤组织中的阳性表达率显著高于卵巢转移性腺癌(P<0.05)。MET4和C-28蛋白阳性表达与卵巢上皮性肿瘤患者的临床病理参数无显著关系。MET4蛋白在细胞质、C-28蛋白在细胞膜的阳性表达与病理分级显著相关(P<0.05)。结论与C-28蛋白比较,MET4蛋白具有更高的特异性和检出率,可能具有更好的临床应用前景。

Objective To evaluate performances of MET4 and C-28 antibody in detection of MET protein expression in ovarian epithelial tumor tissue.Methods Indirect immunohistochemical method was employed to detect MET protein expression in tissue microarray of ovarian epithelial tumor,ovarian metastatic adenocarcinoma and normal ovarian.Analysis of immunostaining was performed by Nuance spectral imaging analytic system.Results Positive staining of MET4 and C-28 protein located in cellular membrane and cytoplasm.Total detection rate of MET4 protein was 64.2%（111/173）,which was higher than that of C-28[50.2%（87/173）]（P＆lt;0.05）.Positive expression rate of MET4 protein in primary ovarian epithelial tumors was significantly higher than that of ovarian metastatic adenocarcinoma（P＆lt;0.05）.Positive expression rates of MET4 and C-28 protein showed no significant correlation with clinicopathological parameters.However,the positive expression of MET4 protein in cytoplasm and C-28 protein in cellular membrane correlated to pathological grading markedly（P＆lt;0.05）.Conclusion Compared to C-28 protein,MET4 protein probably has better prospects for clinical application with higher specificity and detection rate.