adeABC基因对鲍氏不动杆菌耐药性的影响

Effects of adeABC gene expression on drug resistance of Acinetobacter baumannii

目的探讨临床分离鲍氏不动杆菌adeABC基因的表达及其对鲍氏不动杆菌耐药性的作用。方法采用E-test法对临床分离的鲍氏不动杆菌进行耐药性检测,逆转录聚合酶链反应(RT-PCR)检测adeABC基因的表达。结果鲍氏不动杆菌对不同抗菌药物的耐药率如下:哌拉西林97.6%,头孢吡肟60.8%,头孢噻肟75.2%,头孢他啶75.2%,环丙沙星80.0%,左氧氟沙星79.2%,阿米卡星60.8%,庆大霉素50.4%,亚胺培南35.2%,多黏菌素B0.0%,哌拉西林/他唑巴坦48.8%。多重耐药鲍氏不动杆菌的adeB基因携带率为95.0%,调控基因adeR、adeS携带率分别为90%、90%;敏感株的adeB基因携带率为58.1%,调控基因adeR、adeS携带率分别为49.5%、50.4%。结论 adeABC基因表达增强是鲍氏不动杆菌产生耐药性的重要机制。

Objective To discuss adeABC gene expression in clinically separated Acinetobacter baumannii and its effects on drug resistance of Acinetobacter baumannii.Methods E-test method was adopted to clinically separate Acinetobacter baumannii which was subjected to drug resistance testing.Reverse transcriptase-polymerase chain reaction（RT-PCR）was used to detect expression of adeABC gene.Results Drug resistance rates of Acinetobacter baumannii against different antibacterial agents were as follow：piperacillin 97.6%,cefepime 60.8%,cefotaxime 75.2%,ceftazidime 75.2%,ciprofloxacin 80.0%,levofloxacin 79.2%,amikacin 60.8%,gentamicin 50.4%,imipenem 35.2%,polymyxin B 0.0%,piperacillin/tazobactam 48.8%.Carrier rate of adeABC gene in multidrug-resistant Acinetobacter baumannii was 95.0% while those of regulatory gene adeR and adeS were 90%,90%,respectively.Carrier rate of adeB gene in sensitive strains was 58.1% while those of regulatory gene adeR and adeS were 49.5%,50.4%,respectively.Conclusion Enhancement of adeABC gene expression is an important mechanism of drug resistance of Acinetobacter baumannii.