多肿瘤标志物蛋白芯片技术在人群健康体检中的应用

Application of protein microarray technologies for the detection of multiple tumor biomarkers in people's health examination

目的探讨多肿瘤标志物蛋白芯片技术在人群防癌普查中的应用价值。方法采用多肿瘤标志物蛋白芯片技术对4624例接受健康体检的人员进行癌胚抗原(CEA)、甲胎蛋白(AFP)、糖链抗原242(CA242)、CA199、CA125、CA153、神经元特异性烯醇化酶(NSE)、铁蛋白、β人绒毛膜促性腺激素(β-hCG)、游离前列腺特异性抗原(fPSA)、PSA、人生长激素(hGH)12种肿瘤相关抗原物检测。结果 4624例体检人员中筛查出133例血清肿瘤标志物水平升高,其中18例经内窥镜、病理学、影像学检查确诊为早期或中期肿瘤,肿瘤检出率为0.39%。男、女受检者血清CEA、铁蛋白、β-hCG、fPSA、PSA、CA125的差异,<55岁年龄组、≥55岁年龄组受检者血清β-hCG、fPSA、PSA的差异以及肿瘤与非肿瘤受检者血清CA199、NSE、CA242、CEA、AFP、CA125的差异有统计学意义(P<0.05)。结论多肿瘤标志物蛋白芯片检测技术适用于人群的防癌普查。

Objective To discuss the application value of protein microarray technologies for the detection of multiple tumor biomarkers in anti-cancer screening in population.Methods Protein microarray technologies for the detection of multiple tumor biomarkers was employed to detect 12 tumor-associated antigens of 4 624 people who had undergone health examination,such as serum carcinoembryonic antigen（CEA）,alpha-fetoprotein（AFP）,carbohydrate antigen 242（CA242）,CA199,CA125,CA153,neuron-specific enolase（NSE）,ferritin,β human chorionic gonadotrophin（β-hCG）,free prostate specific antigen（fPSA）,PSA,human growth hormone（hGH）.Results Among 4 624 people,serum levels of tumor biomarkers were found to be increased in 133 people,including 18 cases diagnosed as early or mid-term cancer through endoscopy,pathological and imageological examination,with detection rate of 0.39%.Differences of serum CEA,ferritin,β-hCG,fPSA,PSA,CA125 betweeen male and female,β-hCG,fPSA,PSA between ＆lt;55 years old group and ≥55 years old group,as well as CA199,NSE,CA242,CEA,AFP,CA125 between people with tumor and without tumor showed statistical significance（P＆lt;0.05）.Conclusion Protein microarray technologies for the detection of multiple tumor biomarkers is suitable for anti-cancer screening in population.