疑难交叉配血原因的分析与检测策略

Cause analysis of cross-matching difficulties of blood and its detection strategies

目的探索解决疑难交叉配血的检测策略以保证临床输血的安全、有效。方法对交叉配血不合的115例血液标本进行血型检测、直接及间接抗人球蛋白实验。对血型正、反定型不符的标本结合患者的年龄、病史进行血型的进一步检测;对血型正、反定型相符的标本根据直接和间接抗人球蛋白实验的结果进行下一步的分析检测。结果 115例交叉配血不合的标本中血型正、反定型不符30例(26.09%);检出不规则抗体50例(43.48%);检出直接和(或)间接抗人球蛋白实验呈阳性35例。结论根据交叉配血不合的原因,制订适宜的检测策略,可确保临床输血的安全、有效。

Objective To discuss detection strategies to solve cross-matching difficulties of blood and to ensure safety and efficiency of clinical blood transfusions.Methods 115 blood samples with cross-matching difficulties were subjected to blood grouping testing,direct and indirect anti-human globulin testing.Further detections were performed on blood samples with incompatibility positive and negative stereotypes,considering patient’s age and medical history.Further analysis and measurements were undertaken in blood samples with compatibility positive and negative stereotypes according to the results of direct and indirect anti-human globulin test.Results Among 115 blood samples with cross-matching difficulties,incompatibility positive and negative stereotypes were found in 30（26.09%） cases,irregular antibodies in 50（43.48%） cases,and positive results of direct and（or） indirect anti-human globulin test in 35 cases.Conclusion Formulating appropriate detection strategies according to the causes of cross-matching difficulties of blood will guarantee the safety and efficiency of clinical blood transfusion.