特发性血小板减少性紫癜患者PBMC中TRAF2的表达及分析

Increased TRAF2 mRNA expression in PBMC of ITP patients and its clinical implications

目的分析肿瘤坏死因子受体相关因子2(TRAF2)是否参与了特发性血小板减少性紫癜(ITP)的发病机制。方法采用荧光定量FQ-PCR(FQ-RT-PCR)检测了55例首诊ITP患者和45例健康体检者外周血单个核细胞(PBMC)中TRAF2的mRNA表达,分析了其与血小板计数的相关性。对其中12例接受糖皮质激素治疗的患者进行随访,分析治疗2个月以后PBMC中TRAF2的表达变换状况。结果 ITP患者中TRAF2的mRNA表达高于正常对照组(P<0.01),且与血小板计数呈负相关(r=-0.56,P<0.01)。患者接受治疗后,PBMC中TRAF2表达下调。结论 TRAF2在ITP中可能起一定的作用,为以后临床进一步诊治提供一定的帮助。

Objective To identify whether TNF receptor associated factor 2（TRAF2） was involved in the pathogenesis of idiopathic thrombocytopenic purpura（ITP）.Methods The relative expression of TRAF2 in PBMC of 55 ITP patients and 45 healthy controls were detected by FQ-RT-PCR.The relationship between TRAF2 and platelet in ITP patients was analyzed.Twelve ITP patients who received glucocorticoid therapy were followed and relative expression of TRAF2 before and after treatment was compare.Results Increased TRAF2 expression,which negatively correlated with platelet count,was observed in ITP patients.Glucocorticoid therapy decrease TRAF2 expression in ITP patients.Conclusion TRAF2 was involved in the pathogenesis of ITP.