桥本甲状腺炎患者外周血趋化因子CCL20的表达及意义研究

Expression and significance of CCL20 in serum of patients with Hashimoto's thyroiditis

目的:检测桥本甲状腺炎患者外周血中趋化因子CCL20表达水平,探讨CCL20水平与TPOAb和TgAb的相关性。方法:TRIzol提取32例桥本甲状腺炎患者(甲状腺炎组)及22例健康人(对照组)外周血中淋巴细胞总RNA,real-time PCR检测CCL20 mRNA表达水平;用酶联免疫吸附法(ELISA)检测外周血中CCL20蛋白表达水平,统计学方法分析CCL20表达水平与TPOAb和TgAb的相关性。结果:甲状腺炎组外周血中CCL20表达高于对照组(P<0.05);CCL20表达水平与TPOAb和TgAb呈正相关(P<0.05)。结论:桥本甲状腺炎患者外周血中CCL20表达增高,可能参与桥本甲状腺炎的发病。

Objective： To investigate the level of serum CCL20 and its correlation with TPOAb and TgAb.Methods：Thirty-two patients with Hashimoto’s thyroiditis（thyroiditis group） and 22 normal cases（control group） were enrolled in the present study.Total RNA in PBMCs was extracted by using TRIzol.Real-time PCR was used to assay expression level of CCL20 mRNA.CCL20 protein level in serum was detected by ELSIA method.The correlation between CCL20 and TPOAb,TgAb was analyzed using Pearson’s correlation analysis,respectively.Results：Serum CCL20 expression level in thyroiditis group was higher than that in control group.Moreover,the difference between them was statistically significant（P＆lt;0.05）.Serum CCL20 level was positively correlated with serum TPOAb,TgAb,respectively（P＆lt;0.05）.Conclusion：Serum CCL20 expression level increased in patients with Hashimoto’s thyroiditis.CCL20 might be involved in the pathogenesis of Hashimoto’s thyroiditis.