长春市2010年-2012年流感监测情况分析

Analysis of influenza surveillance in Changchun from 2010 to 2012

目的:分析长春市流感监测情况,探索流感流行特点及病原学变化规律,为预防和控制流感大流行提供科学依据。方法:采集门诊流感病人的咽拭子标本用荧光定量PCR,和狗肾细胞(MDCK)及鸡胚法进行流感病毒检测,病毒分离后采用血凝抑制方法(HI)进行流感病毒型别鉴定。结果:从584例流感样病例标本中检出121份核酸阳性并分离到52株流感病毒,分离阳性率为42.98%其中H3N2型13株(占10.74.%),B-victoria系22株(占18.18%),B-yam agata系17株(占14.05%)。而上一年度以甲型为主尤其是以新甲H1型为主。结论:2011年-2012年度长春市流感的流行情况较为平静,H3N2型,B-victoria系,B-yam agata系成为流感流行的优势株,与上一监测年度(新甲H1,H3N2型,B-victoria系)不同。

Objective： To analyze the influenza surveillance situation in Changchun city from 2010 to 2012,explore the influenza epidemic characteristics and etiological changes,and provide scientific basis for prevention and control of influenza pandemic.Methods： The throat swab specimens were collected from outpatient influenza patients and the influenza virus was detected with Real-Time PCR,isolated with MDCK cells and chicken embryo method,and finally identified by hemagglutination inhibition test（HI）.Results： From October 2011 to March 2012,one hundred and twenty-one positive samples of nucleic acid were found from 584 influenza-like cases specimens,and 52 strains of influenza viruses were isolated（42.98%）,including 13 H3N2 strains（10.74%）,22 B-victoria strains（18.18%） and 17 B-yamagata strains（14.05%）.New influenza A（H1） was the main type in last surveillance period.Conclusion： The influenza was found calm in Changchun from 2011 to 2012,and the predominant strains were H3N2,B-victoria and B-yamagata,different from the predominant strains（new A（H1）,H3N2 and B-victoria） of the last surveillance period.