摘要
目的:采用HPLC法测定浮萍中芹菜素的含量。方法:色谱柱:Phenomenex-ODS(4.6mm×250mm,5μm);流动相:甲醇-0.2%磷酸溶液(55:45);检测波长:340nm;流速:1.0ml/min;柱温:40℃。结果:芹菜素在0.101 68～1.016 8μg之间,线性关系良好,加样回收率98.91%,RSD=1.07%(n=6)。结论:该方法操作简便、准确,具有很好的重现性和稳定性,可以用于浮萍的质量控制。
Objective：To determine the Apigenin in Herba Spirodrlae by HPLC. Method：The column employed was Phenomenex -ODS Column（4.6mm×250mm,5μm） ;the mobile phase was methanol -0.2% phosphoric acid （55：45 ）. The flow rate was 1.0ml/min; detection was affected at 340nm and column temperature was 40 ℃. Result ：The linear range of Apigenin was 0.10168-1.0168μg （r = 0. 999 9）, and the mean recovery was 98.91% ,RSD = 1.07% （n =6）. Conclusion：This method is simple, rapid and accurate with good reproducibility. It is suitable for the quality control of Herba Spirodrlae.
出处
《中医药信息》
2013年第3期32-34,共3页
Information on Traditional Chinese Medicine
基金
山东省中医药科技发展计划(2007-009)
关键词
HPLC
浮萍
芹菜素
含量测定
HPLC
Herba Spirodrlae
Apigenin
Determination
