英夫利西单抗对哮喘模型豚鼠抗肿瘤坏死因子-α的影响

Effect of Infliximab on TNF-α of guinea pigs asthma model

目的探讨英夫利西单抗对哮喘模型豚鼠抗肿瘤坏死因子-α(TNF-α)的影响。方法豚鼠50只,雌雄各半,随机分为5组:对照组,模型组,英夫利西单抗低、中、高剂量(5、10、15mg/kg)组,每组10只。模型组和英夫利西单抗各剂量组ip给予10%卵蛋白以致敏,对照组给予等量生理盐水;模型组和英夫利西单抗各剂量组再分两次给予2%卵蛋白溶液雾化吸入,对照组以等量生理盐水替代。每次激发前英夫利西单抗各剂量组ip相应剂量的英夫利西单抗,第二次激发后24h处死动物,对比各组血清、组织匀浆和肺泡灌洗液中TNF-α水平。结果模型组血清、组织匀浆、肺泡灌洗液中TNF-α浓度高于对照组(P<0.05),而英夫利西单抗各剂量组血清、组织匀浆、肺泡灌洗液中TNF-α的浓度低于模型组(P<0.05)。结论TNF-α与豚鼠哮喘形成密切相关;使用英夫利西单抗可以减少豚鼠哮喘模型血清、组织匀浆、肺泡灌洗液中TNF-α的产生。

Objective To investigate the effect of Infliximab on TNF-α of guinea pigs asthma model.Methods Guinea pigs（50）,half male and female,were randomly divided into five groups,control,model,low-,mid-,and high-dose Infliximab（5,10,and 15 mg/kg）groups（n=10）.Model and the Infliximab groups were ip administered with 10% ovalbumin to sensitivate,and the control group was given physiological saline.Model and Infliximab groups were atomizing inhalated twice with 2% ovalbumin solution,and the control group was given the same dose physiological saline.Infliximab groups were ip administered with appropriate doses of Infliximab before excitation each time,and animals were killed 24 h after the second excitation.TNF-α was detected in serum,tissue,and bronchoalveolar lavage fluid.Results TNF-α concentration in the serum,tissue homogenates,and bronchoalveolar lavage fluid in the model group was higher than that in the control group（P ＆lt; 0.05）.However,TNF-α concentration in the serum,tissue homogenates,and bronchoalveolar lavage fluid of the Infliximab group was lower than that in the model group（P ＆lt;0.05）.Conclusion TNF-α is related to the formation of the guinea pig asthma,and Infliximab could reduce TNF-α of serum,tissue homogenates,and bronchoalveolar lavage fluid in guinea pig model of asthma.