正交试验-多元回归法优化葛根的乙醇提取工艺

Optimization of extract technology with alcohol for Puerariae Radix by orthogonal test-multiple regression method

目的优化葛根药材中葛根素的提取工艺。方法采用乙醇为溶媒提取葛根药材,以葛根素转移率和出膏率为指标,运用正交试验及多元回归分析多指标综合优化葛根药材的提取条件。结果葛根提取的最佳条件为:90%乙醇提取两次,每次6倍量乙醇,提取时间为2h。葛根素的转移率为89.85%,浸膏得率为14.92%。结论该数据处理方法优化葛根提取工艺结果准确、高效,具有一定的推广价值。

Objective To seek the best way for extracting puerarin from Puerariae Radix.Methods Puerariae Radix was extracted by ethanol,using the transfer rate of puerarin and extract rate as indexes.Based on the orthogonal test combined with multi-variable regression analysis to make sure the best way for extracting puerarin from Puerariae Radix.Results Puerariae Radix should be extracted by 90% ethanol twice with six times of ethanol and 2 h each time.The transfer rate of puerarin was 89.85% and extract rate was 14.92%.Conclusion The analytical method of the orthogonal data is accurate and efficient to optimize the preparation of puerarin from Puerariae Radix.