顶空气相色谱法测定一次性使用输液器中环己烷残留量

Determination of cyclohexane residues in disposable infusion set by headspace gas chromatography

目的采用顶空气相色谱法对一次性使用输液器中黏合剂环己烷的残留进行检测。方法色谱柱为HP-5石英毛细管柱(30m×0.32mm×0.25μm),柱温采用程序升温,检测器为氢火焰离子化检测器,检测器温度为250℃,进样口温度为130℃,分流比为20:1,以15%乙醇为溶解介质。结果该方法的检测限可达12ng/mL,检测浓度的线性范围为0.10～50.57μg/mL(r=0.9995),平均回收率为93.6%。结论本方法简单、快速、准确性高、重复性好,能够满足一次性使用输液器中环己烷残留量检测的需要。

Objective To establish the method for the determination of adhesion agent cyclohexane residues in disposable infusion set by headspace gas chromatography.Methods The residual solvents were analyzed on HP-5 capillary column（30 m × 0.32 mm × 0.25 μm）using temperature program.FID was used as a detector with the temperature at 250 ℃.The inlet temperature was 130 ℃,the split ratio was 20：1,and 15% alcohol was used as the solvent.Results The determination limit was 12 ng/mL.The linear range of cyclohexane was 0.10 — 50.57 μg/mL（r=0.999 5）.The average recovery was 93.6%.Conclusion The method is simple,fast,accurate,and reproducible,so it can be used for the determination of residual solvent in disposable infusion set.