缺氧诱导因子-1α诱导型一氧化氮合酶在胃癌中的表达研究

Hypoxia-inducible factor-1α,inducible nitric oxide synthase expressed in Human gastric cancer

目的研究缺氧时胃癌组织表达缺氧诱导因子-1α(HIF-1α)及诱导型一氧化氮合酶(iNOS)的变化,探讨HIF-1α、iNOS与胃癌组织分化、淋巴结转移等生物学行为特征的关系。方法采用免疫组织化学技术检测45例胃癌及10例癌旁正常组织中HIF-1α、iNOS蛋白质的表达。结果 45例胃癌中HIF-1α、iNOS蛋白阳性表达率分别为66.7%(30/45)、75.6%(34/45)。HIF-1α蛋白阳性表达率与癌组织分化程度、浸润深度、淋巴结转移和TNM分期显著相关,而iNOS蛋白阳性表达率与癌组织浸润深度、淋巴结转移和TNM分期显著相关,HIF-1α与iNOS表达一致符合率为57.7%(26/45),两者表达显著相关。结论胃癌组织存在HIF-1α蛋白的过表达,HIF-1α诱导iNOSmRNA及蛋白表达,协同促进肿瘤发生发展。HIF-1α、iNOS的测定可作为判断胃癌转移潜能的重要指标。

Objective Hypoxia is a common phenomenon in the tumor microenviroment of most solid tumours, inclu-ding human gastric cancer. Hypoxia-inducible factor-lα（ HIF-1α） is universally expressed in many types of alls, and plays a key role in modulation of tumour related genes under hypoxia. Our research was to detect protein expres-sion of HIF-1 α and iNOS, and explore its relationship with biological characteristics of gastric cancer, such as turnout angiogenesis, tomour differentiation, and metastasis. Method Immunohistochemistry was used to detect the expres-sion of HIF-1α, iNOS and MVD in 45 resected specimens of gastric cancer and 10 paracancerous tissues. Result The expression of HIF-1α and iNOS protein were 66.7% （30/45）,75.6% （34/45）in gastric cancer. The expres- sion of HIF-1 α protein were significantly correlated with differentiation, infiltration, metastasis and TNM. While the expression of iNOS protein were significantly correlated with, infiltration, metastasis and TNM. The coincidence rate of the expression of HIF-1 α and iNOS was 57.7% （26/45） showing a close relationship between both of them. Conclusion HIF-1 α protein expressed overly in gastric cancer. The expression of iNOS mRNA and protein is in-duced by HIF-1α protein and they have a synergistic effect in the neovascularization of the tumour. The expression of HIF-1α and iNOS protein can be indicators for the metastasis in gastric cancer.