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介孔TiO_2晶须材料的硅烷化改性及其对载体pzc值和酶负载性能的影响 被引量:4

Effect of surface modification with silane coupling agent on enhancing pzc value and enzyme loading capacity of mesoporous TiO_2 whiskers
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摘要 以3-氨丙基三乙氧基硅烷(APTES)对介孔二氧化钛晶须(MTiO2_ws)进行表面修饰,使其电荷零点值(pzc)由5.3提高至6.8,改性后材料MTiO2_ws-APTES的比表面积略有上升,但孔结构基本不变。以MTiO2_ws-APTES为载体对γ-谷氨酰转肽酶(GGT)进行了固定化,当给酶量小于150U.g-1时,酶活回收率均大于99%,固定化酶MTiO2_ws-APTES-GGT的比活力最高可达184.0U.g-1,对酶的负载性能显著优于MTiO2_ws。相比于游离酶,MTiO2_ws-APTES-GGT的最适温度和热稳定性均略有下降,但pH稳定性明显优于游离酶和以MTiO2_ws为载体的固定化酶(MTiO2_ws-GGT);MTiO2_ws-APTES-GGT对γ-谷氨酰对硝基苯胺(GpNA)的亲和力常数(Km)为0.889mmol·L-1,较游离酶有所上升,但小于MTiO2_ws-GGT。MTiO2_ws-APTES-GGT的稳定性良好,经4℃下储藏60d,连续使用21批次后残余酶活仍可达初始值的80.07%。 Mesoporous TiO2 whiskers (MTiO2 _ ws)were chemically modified with 3-aminopropyltriethyloxy silane (APTES). As a result, the point of zero charge (pzc) for the modified material (MTiO2 _ ws- APTES) rose from 5.3 to 6.8. Also the specific surface area of MTiO2 _ ws-APTES slightly increased, but the pore structure had little change compared to that of MTiO2 _ ws. Then, MTiO2 _ ws-APTES was used as the support for γ-glutamyltranspeptidase (GGT) immobilization. MTiO2 _ ws-APTES showed better loading capacity than MTiO2 _ ws. While the enzyme concentration was less than 150 U . g- 1, the recovery of activity was above 99% and the highest specific activity for immobilized GGT (MTiO2 _ ws APTES-GGT) was 184.0 U . g -1. Although both optimal temperature and thermal stability of MTiO2 _ws-APTES-GGT slightly decreased compared with the native enzyme, pH stability was greatly improved. The affinity constant (Kin) of MTiO2 _ ws-APTES-GGT toward γ-glutamyl-p-nitroaniline (GpNA) was also determined to be 0. 889 mmol . L -1, which was higher than that of the native enzyme, but less than that of MTiO2 _ ws-GGT. After storage at 4℃ for 60 days and reused for 21 batches, MTiO2 _ ws-APTES-GGT maintained 80.07% of its initial activity.
出处 《化工学报》 EI CAS CSCD 北大核心 2013年第6期2160-2168,共9页 CIESC Journal
基金 江苏省高校自然科学研究重大基金项目(10KJA530014) 江苏省科技支撑计划(社会发展)基金项目(SBE 201078182) 江苏省自然科学基金项目(BK2011799 BK2012825)
关键词 介孔氧化钛晶须 化学改性 3-氨丙基三乙氧基硅烷 固定化酶 稳定性 担载量 mesoporous TiO2 whiskers chemical modification 3-aminopropyltriethyloxy silane enzyme immobilization stability loading capacity
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参考文献20

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二级参考文献17

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