摘要
目的优化测定绵萆薢中薯蓣皂苷元含量的高效液相色谱法,为评价绵萆薢药材质量提供可行的方法。方法色谱柱为symmetryshield TMRP18(150.0mm×3.9mm,5μm),以乙腈-水(80∶20)为流动相,流量1.2mL/min,检测波长203nm,柱温为常温。结果薯蓣皂苷元进样量在0.019 8~0.505 0g/L范围内线性关系良好(r=0.999 5),平均回收率为98.23%。结论本方法操作简便、准确,可用于绵萆薢的质量控制。
Objective To optimize an HPLC method for determination of Diosgenin in Dioscorea septemloba Thunb and provide a basis for assessing the quality of this drug. Methods The column was symmetryshield TMRP18 (150.0 mm×3.9mm,5μm) ;The mobile phase consisted of acetonitrile'water (80 : 20) at a flow rate of 1.2 mL/min; The determination wave length was set at 203 nm and the column temperature was room temperature. Results The linear range of Diosgenin was 0.019 8-- 0.505 0 g/L (r=0.999 5), the average recovery was 98.23%. Conclusion The established method is simple, convenient and ac- curate, which can be utilized for quality control of Dioscorea septemloba Thunb.
出处
《青岛大学医学院学报》
CAS
2013年第2期124-125,128,共3页
Acta Academiae Medicinae Qingdao Universitatis
关键词
绵萆薢
薯蓣皂苷元
色谱法
高压液相
质量控制
diascorea septemloba thunb
Diosgenin
chromatography
high pressure liquid
quality control