基于紫外光谱的丹红提取液质量快速分析技术

Application of ultraviolet spectroscopy for rapid analysis in extraction process of Danhong injection

该文建立了一种基于紫外光谱的丹红提取液质量快速分析技术,用于测定丹红提取液中丹参素、迷迭香酸、紫草酸、丹酚酸B和羟基红花黄色素A等5个成分的含量。在丹参和红花药材的提取过程中取样得到提取液样品44个,扫描紫外光谱,并用HPLC测定5个成分含量。使用偏最小二乘法(PLS)建立紫外光谱与5个成分含量之间的多元校正模型。结果表明,建立的模型能较好地预测丹红提取液中5个成分的含量。该方法能快速分析丹红注射液生产过程中间体的质量,为丹红注射液生产全程质量控制提供了方法。

In this work, a rapid analysis method basing on ultraviolet spectroscopy was established for the determination of danshensu, protocatechuic aldehyde, rosmarinci acid, lithospermic acid and salvianolic acid B in the extraction process of Danhong injection. In the extraction process of Danshen and Honghua crude drugs, 44 extraction solution samples were collected and the contents of the five components were determined by HPLC analysis. The ultraviolet spectra of the samples were collected. Partial least square regression was used to establish the multivariate calibration models between the ultraviolet spectra and the contents of the five components. The results showed that the established models could predict the contents of the five components in the extraction solution accurately. The ultraviolet spectroscopy method established in this work can be used for rapid analysis of the intermediates of Danhong injection, which may be applied for the quality control in the manufacturing process.