茶多酚对白癜风患者CD8＋T淋巴细胞分泌肿瘤坏死因子α、干扰素γ和白介素2受体影响

In vitro effects of tea polyphenols on the secretion of TNF-a, IFN-y and IL-2R by peripheral blood CD8＋ T lymphocytes from vitiligo patients

目的检测CD8＋T淋巴细胞分泌细胞因子及受体在白癜风患者外周血中的表达情况及意义，并探讨茶多酚对其表达的影响。方法分离和培养12例进展期白癜风、12例稳定期患者和10例健康人外周血CD8’T淋巴细胞，与茶多酚100mg／L共培养，酶联免疫吸附试验（ELISA）检测茶多酚处理前后肿瘤坏死因子（TNF）-a、干扰素（IFN）-1和白介素-2受体（IL-2R）的浓度。用t检验和方差分析检测不同组间及处理前后各因子水平的差异。结果进展期白癜风组、稳定期白癜风组和健康对照组外周血CD8＋T淋巴细胞分泌TNF—a的浓度依次降低，分别为（191．302±6．077）ng／L、（175．966±2．467）ng／L、（173．664±3．600）ng／L，IFN-Y的浓度依次升高，分别为（280．182±36．07）ng／L、（371．670±24．352）ng／L、（447．147±8．432）ng／L，IL-2R的浓度同样依次升高，分别为（8．375±0．161）μg/L、（8．845±0．161）μg/L、（9．345±0．125）μg／L。进展期白癜风组与稳定期白癜风组和健康对照组相比较，3种指标差异均有统计学意义（P〈0．05或0．01）。经茶多酚处理2d后，进展期白癜风组、稳定期白癜风组和健康对照组TNF—d水平均明显降低至（164．797±1．784）ng／L、（166．150±3．576）ng／L、（155．028±5．759）ng／L，与处理前比较差异均有统计学意义（均P〈0．05）；稳定期白癜风组IFN-1浓度升高，而进展期白癜风组和健康对照组则有降低，各组IL-2R水平均有升高，但差异均无统计学意义（均P〉0．05）。结论白癜风患者外周血CD8’T淋巴细胞分泌的细胞因子及受体的变化可能与白癜风诱发或进展相关。茶多酚可能通过降低CD8’T淋巴细胞分泌TNF-仪治疗白癜风。

Objective To detect the level of cytokines and their receptors secreted by peripheral blood CD8＋ T lymphocytes from vitiligo patients, and to evaluate the influence of tea polyphenols on their secretion. Methods CD8＋ T lymphocytes were isolated from the peripheral blood of 12 patients with progressive vitiligo, 12 patients with stable vitiligo and 10 healthy controls, and cultured in vitro for 20 days. Then, some CD8＋ T lymphocytes were treated with tea polyphenols of 100 mg/L for two days. Enzyme-linked immunosorbent assay （ELISA） was carried out to determine the levels of tumor necrosis factor （TNF）-a, interferon （IFN）-a and interleukin-2 receptor （IL-2R） in the culture supernatants of CD8＋ T lymphoeytes before and after the treatment with tea polyphenols. Analysis of variance and t test were conducted to assess differences in these parameters among these groups and changes between pretreatment and posttreatment, respectively. Results There was a sequential decrease in the level of TNF-a （（ 191.302±6.077） vs. （175.966± 2.467） vs. （173.664 ± 3.600） ng/L, F = 4.784, P 〈 0.05）, bat a sequential increase in that of IFN-a （ （280.182 ± 36.070） vs. （371.670 ± 24.352） vs. （447.147 ± 8.432） ng/L, F= 9.036, P〈 0.01） and IL-2R （（8.375 ± 0.161） vs. （8.845 ±0.161） vs. （9.345 ± 0.125） ng/L, F = 9.639, P 〈 0.01） in the culture supernatant of CD8＋ T lymphocytes from patients with progressive vitiligo to patients with stable vitiligo and healthy controls. After two days of tea polyphenol treatment, a statistical decrease was observed in the level of TNF-a in the culture supematant of CD8 ＋ T lymphocytes from patients with progressive vitiligo （（ 164.797 ± 1.784） ng/L, P 〈 0.01 ）, patients with stable vitiligo （（166.150 ± 3.576） rig/L, P 〈 0.05） and healthy controls （（155.028 ± 5.759） ng/L, P 〈 0.05）, but no statistical changes were noted for IFN-＇y or IL-2R （all P 〉 0.05） despite a slight elevation in the level of IFN-＇y in the culture supernatant of CD8＋ T lymphocytes from patients with stable vitiligo and in that of IL-2R from all the three groups as well as a mild reduction in that of IFN-Y from patients with progressive vitiligo and healthycontrols. Conclusions The changes of cytokines and their receptors secreted by CD8＋ T lymphocytes may be associated with the induction and progression of vitiligo. Tea polyphenols may treat vitiligo via suppressing the secretion of TNF-a by CD8＋ T lymphocytes.