正弦交变电磁场对体外培养大鼠股骨骨组织的影响

Effect of Sinusoidal Electromagnetic Fields on Rats Femur Tissue Cultivation in Vitro

研究50Hz、1.8mT正弦交变电磁场(SEMFs)对体外培养大鼠股骨组织的影响。体外分离培养大鼠股骨组织,随机分为两组(磁场处理组和对照组)。磁场处理组用1.8mT的SEMFs每天处理股骨组织1.5h。在SEMFs处理后的第0d(第一次处理1.5h后)、1、2、3、4和5d,采用Real-time RT-PCR检测培养股骨组织中骨形成相关基因表达量的变化;在SEMFs处理后的第3、6、9和12d测定培养股骨组织中碱性磷酸酶(ALP)活性变化;在SEMFs处理后的3、6、9和12d测定培养股骨中钙含量的变化。结果表明,SEMFs能使OPG和Collagen-1mRNA表达量保持在一个相对稳定的水平,在SEMFs处理后第1d和5d明显增加Runx-2 mRNA的表达水平。在SEMFs处理后的3d和9dALP活性明显增高(P<0.01)。SEMFs处理培养股骨组织6、9和12d后,钙含量明显高于对照组(P<0.01)。50Hz、1.8mT SEMFs能促进体外培养大鼠股骨组织中骨代谢相关基因的表达;增加钙含量以及ALP活性。因此,本实验从体外培养股骨骨组织水平提供了SEMFs促进骨代谢的实验依据。

The present research was aimed to investigate the effect of 50Hz, 1.8mT of sinusoidal electromagnetic fields（SEMFs）on femur tissue cultivation in vitro. The rat femur tissue was isolated from SD rats by method of enzyme digestion, and randomly divided into two groups： SEMFs group and control. The femur tissue of SEMFs groups were exposured under 50Hz 1.8mT of SEMFs for 1.5h/time/d, but those in the control groups were without SEMFs treatment. The correlative gene was detected by the Real-time RT-PCR that after SEMFs treatment for 0 （first times treatments is 0 days）, 1, 2, 3, 4 and 5d. The alkaline phosphatase （ALP） activity was measured after SEMFs treatment for 3, 6, 9 and 12d respectively. The calcium content was detected after SEMFs treatment for 3, 6,9 and 12d. The results showed that the OPG and Collagen-1 mRNA expression level was kept at a relatively stable level by SEMFs in the SEMFs group significantly. The Runx-2 mRNA expression level was significantly increased after the SEMFs treatment for ld and 5d. The ALP activity of femur tissue was significantly increased after SEMFs treatment for 3d and 9d. The calcium content was higher than untreated groups after SEMFs treatment for 6, 9 and 12d. The SEMFs promoted OPG, Collagen-1, Runx-2 mRNA expression level, ALP activity and calcium content. The result indicated that SEMFs increased the metabolism activity of femur tissues.