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Runx1转基因小鼠构建

Construction of Runx1 Transgenic Mice
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摘要 本研究目的在于构建Runx1转基因小鼠模型。利用含小鼠Runx1cDNA的ptetO7-Runx1-AscⅡ-IRES-EGFP表达载体,应用基因重组技术和显微注射技术,将纯化的线性化表达质粒ptetO7-Runx1-AscⅡ-IRES-EGFP注射进受精卵的原核内,将存活受精卵或存活胚胎移植制备Runx1转基因小鼠,用PCR技术和Southern blot实验方法鉴定转基因小鼠是否构建成功。实验结果表明本方法可成功构建携带Runx1基因的转基因小鼠模型,为进一步研究Runx1基因的生物学功能奠定了基础。 This study was aimed to construct transgenic mouse model with target for Runxl gene. Runxl cDNA of mice was amplified by PCR from pcDNA3.1 Flag Runxl FL vector and inserted into ptetO7-Asc-IRES-EGFP vector to form a recombinant vector, and then the recombinant vector was injected into fertilized egg by microinjection tech nology to get a transgenic mouse. The results of PCR and Southern blot indicated that the Runxl transgenic mouse was constructed successfully, and this could provide an important tool for studying the function of Runxl gene in vivo.
作者 汤小菊 李君丽 刘小菁 雷弋 苏巧俐 吴庆波 罗凤鸣
机构地区 四川大学华西医院再生医学研究中心心血管疾病研究室 四川大学华西医院全科医学科
出处 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2013年第3期584-587,共4页 Journal of Biomedical Engineering
基金 国家自然科学基金资助项目(30871118 30971325 81270129 11072163) 四川省科技厅项目资助(2009SZ0190) 成都市科技局项目资助(11DXYB095SF)
关键词 载体构建 RUNX1 转基因小鼠 Vector construction Runxl Transgenic mouse
分类号 Q78 [生物学—分子生物学]
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生物医学工程学杂志
2013年 第3期

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