减重步行训练结合督脉电针对脊髓损伤大鼠运动功能和脑源性神经营养因子的影响

Effects of body weight support treadmill training combined with Du Meridian electroacupuncture on loco motion function and expression of brain-derived neurotrophic factor in rats with spinal cord transection

目的:研究减重步行训练、督脉电针及两者联合治疗对横断性脊髓损伤大鼠运动功能及脑源性神经营养因子(BDNF)表达的影响。方法:对72只成年SD大鼠建立胸10脊髓横断损伤模型,随机分为对照组、减重步行训练组(BWSTT)、督脉电针组(电针组,EA)和训练+电针(联合组,BWSTT+EA)各18只,每组再分为8d、15d和30d3个小组(n=6)。对各组大鼠进行BBB评分并用免疫组织化学法测定脊髓损伤尾端组织中BDNF的表达。结果:单纯减重步行训练或/和督脉电针均能使不同时间点脊髓损伤大鼠BBB评分、脊髓组织BDNF表达水平提高(P<0.05);减重步行训练和督脉电针对BBB评分、脊髓组织BDNF表达存在交互作用(P<0.05),提示减重步行训练和督脉电针联合干预对促进脊髓损伤的康复效果更佳。各治疗组随着干预时间的增加表现出不同程度的BBB评分、脊髓组织BDNF表达水平提高(P<0.01);尽量长时间的应用减重步行训练和督脉电针的联合治疗对促进横断脊髓损伤大鼠运动功能恢复的疗效最佳。结论:减重步行训练结合督脉电针可以促进横断性脊髓损伤大鼠的运动功能恢复,干预时间越长疗效越佳,且3者间存在协同作用,这种运动功能恢复可能与脊髓组织中BDNF的增加有关。

Objective： To study the effects of body weight support treadmill training（BWSTT） or Du Meridian electroacu- puncture （EA） or BWSTT＋ EA （combination therapy） on locomotion function and expression of brain-derived neurotrophic factor（BDNF） in rats with spinal cord transection. Method： A total of 72 adult SD rats were divided into control group, BWSTT group, EA group and BWSTT＋ EA group, with 18 rats in each group, and 3 subgroups（n=6） for 8d,15d and 30d respectively. Spinal cord tran- sections were performed at T10 level. Their motor function were assessed with Bosso-Beattie-Bresnahan locomo- tor rating scale （BBB scale） for open field and the expressions of BDNF in spinal cord tissues to the injury caudal ends were measured with immunohistochemistry. Result： The scores of BBB scales and levels of BDNF increased after BWSTT or/and EA therapy in different time point respectively;and there was interaction between BWSTT and EA for the increases of scores of BBB scales and levels of BDNF, The combination therapy of BWSTT and EA was better in promoting restoration of motor function for spinal cord injury（SCI） rats. And the BBB scales and expressions of BDNF in each therapy group increased with longer duration of therapy（P〈0.01）;Applications of combination therapy of BWSTT and EA as long duration as possible obtained the best effect on locomotion recovery in the transected SCI rats. Conclusion：The combination therapy of BWSTT and EA for longer duration have synergistic effect on SCI rats locomotion recovery which may associate with the increases of BDNF in injured spinal cord.