甲状腺相关眼病患者血清中白细胞介素-15和白细胞介素-21的含量及临床意义

Contents and clinical significances of interleukin-15 and interleukin-21 in serum of thyroid-associated ophthalmopathy patients

目的探讨甲状腺相关眼病(thyroid-associated ophthalmopathy,TAO)患者血清中白细胞介素-15(interleukin-15,IL-15)和IL-21的含量变化及其临床意义。方法收集2011年6月至2012年4月于我院诊断为TAO患者的资料及血清样本76例,参照TAO临床活动度评分(clinicalactivityscore,CAS)标准将患者分为非典型活动期(CAS<4分)组(33例)和活动期(CAS≥4分)组(43例),根据TAO眼部改变并结合CAS将其又分为非浸润组(30例)和浸润组(46例)。另收集同期正常体检血清标本35例作为正常对照组。采用酶联免疫吸附实验(ELISA)检测各组血清中IL-15和IL-21的含量。结果 TAO活动期组患者血清中IL-15和IL-21的含量分别为(389.42±278.32)pg·mL-1、(248.54±157.32)pg·mL-1,TAO非活动期组分别为(45.06±8.54)pg·mL-1、(12.62±6.47)pg·mL-1,正常对照组分别为(26.67±5.86)pg·mL-1、(7.58±5.49)pg·mL-1。TAO活动期组患者血清中IL-15和IL-21的含量均明显高于TAO非活动期组和正常对照组,差异均有显著统计学意义(均为P<0.01);TAO非活动期组患者血清中IL-15和IL-21的含量高于正常对照组,差异均有统计学意义(均为P<0.05)。浸润性TAO组患者血清中IL-15和IL-21的含量分别为(358.12±248.51)pg·mL-1、(218.45±119.42)pg·mL-1,均明显高于非浸润性TAD组的(29.06±6.79)pg·mL-1、(7.79±5.58)pg·mL-1和正常对照组,差异均有显著统计学意义(均为P<0.01);非浸润性TAO组患者血清中IL-15和IL-21含量稍高于正常对照组,但差异均无统计学意义(均为P>0.05)。结论血清中IL-15和IL-21的含量检测可用于评估TAO的炎症活动性和浸润情况。

Objective To investigate the contents and clinical significances of in terleukin-15 （ IL-15 ） and IL-21 in serum of thyroid-associated ophthalmopathy （TAO） pa tients. Methods A total of 76 TAO patients with their data and blood samples were collected in our hospital from June 2011 to April 2012 ,then they were divided into TAO inactive period group （ CAS 〈 4 points, 33 cases） and TAO active period group （ CAS 〉 4 points,43 cases） by TAO clinical activity score standard, and were divided into non in vasive TAO group （30 cases） and invasive TAO group （46 cases） by eye complications combined with CAS. Another 35 normal cases with their blood samples were collected as normal group. The content of IL-15 and IL-21 in serum of patients were detected by ELISA. Results The content of IL-15 and IL-21 in serum of TAO active period group were （ 389.42 ± 278.32 ） pg · mL - 1 and （ 248.54 ± 157.32 ） pg · mL 1, TAO inactive period group were（45.06 ± 8.54）pg · mL-1 and（ 12.62 ± 6.47 ） pg · mL-1 , and normal control group were （26.67 ± 5.86） pg · mL- 1 and （ 7.58 ± 5.49） pg · mL- 1. As to the content of IL-15 and IL-21 in serum, TAO active period group were both significantly obviously higher than those of TAO inactive period group and normal control group（ all P 〈 0.01 ）, and TAO inactive period group were both significantly higher than those of normal con-trol group（both P 〈 0.05 ） ;Invasive TAO group were both significantly obviously higher than those of non invasive TAO group and normal control group（ all P 〈 0.01 ） ; Non invasive TAO group were both significantly a little higher than those of normal control group （ both P 〈 0.05 ）. Conclusion The content detection of IL-15 and IL-21 in serum of TAO patients can be used to evaluate the situation of inflammation activities and infiltration.