苏拉明对小鼠肺腺癌瘦素表达的调控及意义

Regulatory effect of Suramin on expression of leptin in transplanted lung adenocarcinoma in mice and its significance

目的观察苏拉明对肺腺癌小鼠异体移植瘤生长的抑制作用,通过检测移植瘤内瘦素(leptin)的表达,初步探讨苏拉明抑制肿瘤生长的相关机制。方法构建肺腺癌小鼠移植瘤模型,将24只接种Lewis肺癌的C57BL/6J小鼠按随机数字表法随机分成3组:对照组、顺铂组和苏拉明组。给予相应药物干预,于接种后24日处死各组小鼠,剥离皮下肿瘤,计算抑瘤率,免疫组织化学检测皮下移植瘤中leptin和血管内皮生长因子(VEGF)的表达并定量分析。结果顺铂组、苏拉明组抑瘤率分别为39.2%、49.1%,与对照组比较,瘤质量差异有统计学意义(P<0.01),对照组、顺铂组和苏拉明组leptin灰度值分别为135.47±5.30、127.87±5.51、80.44±4.69;VEGF灰度值分别为156.26±6.45、134.7±5.81、96.13±5.66。结论苏拉明对肺腺癌移植瘤的生长具有明显抑制作用,其机制可能是通过抑制leptin过表达,下调VEGF表达抗肿瘤血管生成,抑制肿瘤的生长。

Objective To observed the inhibition of Suramin on transplanted lung adenoearcinoma in mice by detecting the expression of leptin in transplanted tumor, and to understand the anti-tumor effects of Suramin. Methods Mouse model of transplanted lung adenocarcinoma was built. Twenty-four C57BL/6J mice inoculated with Lewis lung carcinoma were randomly divided into three groups using random number table ： control group, cisplatin group and suramin group. After the appropriate drug intervention, mice in each group were sacrificed 24 hours after inoculation. Subcutaneous tumor was peeled off and the inhibitory rate was calculated. The leptin and vascular endothelia growth factor （VEGF） were detected by immunohistochemical methods. Results The inhibition rate of tumor in cisplatin group and Suramin group was 39.2% and 49.1%, and the tumor weight was significantly different compared with that of control group （ P 〈 0.01 ） , The grayscale values of leptin in control group, cisplatin group and the Suramin group were 135.47 ± 5.30,127.87 ± 5.51 and 80.44 ± 4.69, VEGF gray values were 156.26 ± 6.45,134.7 ± 5.81 and 96. 13 ±5.66. Conclusions Suramin can significantly inhibit tumor growth in transplated lung adenocarcinoma. The mechanism may be inhibition of leptin and VEGF expression.