成都地区人乳头瘤病毒16型E2基因和长控制区序列分析

Analysis of human papillomavirus 16 subtypes E2 gene and long control region sequence in Chengdu

目的研究人乳头瘤病毒（HPV）16型E2基因和长控制区（LCR）序列变异以及其与子宫颈病变的相关性。方法成都地区HPVl6型感染者标本50份，包括38份HPV携带者的子宫颈脱落细胞，8份生殖器疣患者的疣体组织，2份子宫颈上皮内瘤变（CIN）Ⅱ级和2份子宫颈CINⅢ级的病变组织。PCR扩增E2基因与LCR序列并测序，构建进化树。结果50份标本中，E2基因有12个突变位点，其中1份生殖器疣标本3683有C→A突变；其他48份标本均存在≥2个位点的突变。所有标本的LCR序列都有突变，共有28个突变位点。选择10份标本测序并构建进化树，8份为Asian变异株；E2突变在各种子宫颈病变中均存在，而LCR7867G→A为4份子宫颈CIN所特有。结论LCR7867G→A突变是成都地区子宫颈病变相关的一个突变位点。

Objective To explore the relevance between sequence variation of human papillomavirus （HPV）16 subtypes E2 gene or long control region （LCR） and cervical lesions. Methods Fifty specimens from HPV16 infected people in Chengdu were collected, including cervical exfoliated cells from 38 HPV carriers, papilloma tissues from 8 cases of genital warts, 2 with cervical intraepithelial neoplasia （CIN） Ⅱ and 2 with CIN Ⅲ in this study. Polymerase chain reaction was used to amplify E2 gene and LCR, then an evolutionary tree was constructed. Results In all the 50 specimens, there were 12 mutation sites in E2 gene, among which, C→A existed in one specimen of genital warts, and ≥2 mutation sites existed in all the other 48 specimens. There were 28 mutation sites of LCR sequence of all the specimens. Ten specimens were chosen to construct evolutionary tree and were sequenced. The data showed that 8 specimens were Asian variants, E2 gene mutation existed in all the specimens while the LCR 7867 G→A only existed in the four CIN. Conclusion LCR 7867 G→A is a correlative mutation site of cervical lesions in Chengdu.