雌激素受体基因多态性与慢性乙型肝炎易感性的相关性

Relationship between ERa and ERβ Gene Polymorphisms and Susceptibility to Chronic Hepatitis B

目的探讨人群中和ERβ基因多态性与慢性乙型肝炎易感性之间的关系。方法选取112例慢性乙型肝炎患者和100例健康对照者，采用聚合酶链式反应-限制性片段长度多态性（PCR—RFLP）分析法检测该人群中ERa和ERβ基因多态性。结果与对照组比较、慢性乙型肝炎组ERa各基因型的频率分布有差异，具有统计学意义；与对照组比较，慢性乙型肝炎组ERβ各基因型的频率分布无差异，没有统计学意义。经Logistic回归分析，与携带肼dPvuⅡ C／C者比较．携带ERaPvu II C／T者发生慢性乙型肝炎的危险性为OR：1．86（95％CI：1．077～3．213）；与携带ERaXbnIGIG者比较，携带ERaXbaIA／G者发生慢性乙型肝炎的危险性为OR=4．2（95％CI：2．360～7．475）：结论ERdnMⅡ、职“XbaI基冈多态性与慢性乙型肝炎的易感性相关：EPq3RsaI、ERβAlu I基因多态性与慢性乙型肝炎的易感性没有相关性．

Objective To examine the polymorphisms of ERo~ and ER[3 genes and their association with susceptibility to chronic hepatitis B. Methods A total of 112 patients with chronic hepatitis B and 100 healthy controls were genotyped for ERa Pvu II , ERa Xba I , ER[3Rsa I and EPβAlu I gene polymorphisms by using the polymERase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method. Results The results showed that there was a significant difference in the frequency distribution of ERa genes rather than EPβ genes between chronic hepatitis B patients and healthy controls （ P 〈 0.05 ） . Multivariate logistic regression analysis showed that the subjects carrying the ERa Pvu II C/T genotype were at a 2-fold elevated risk for chronic hepatitis B （OR = 1.86, 95% CI： 1. 077 -3. 213） compared to those carrying ERolPvu II C/C. The subjects carrying the ERa Xba I A/A genotype were at a 4-fold elevated risk for chronic hepatitis B （ OR = 4.2, 95 % CI ： 2. 306 - 7. 457） compared to those carrying ERa Xba I G/G. Conclusion The polymorphisms of ERa Pvu II and ERa Xba I genes instead of ERI3 Rsa I and EPq3 Alu I genes are closely associated with susceptibility to chronic hepatitis B.