毛发生长周期对小鼠皮肤创伤愈合的影响

Impact of hair growth phase on mouse skin wound healing

目的探讨处于不同毛发生长周期的C57BL/6小鼠皮肤创伤愈合速度。方法制备小鼠皮肤创伤模型,计算术后0、3、7 d创面愈合率,愈合率=(原始创面面积-未愈合的创面面积)/原始创面面积×100%,比较毛发静止期(Hair telogen stages)小鼠和毛发生长期(Hair anagen stages)小鼠伤口愈合速度。采用HE染色比较伤口愈合的组织形态结构差异,利用BrdU检测伤口周围细胞增殖。结果毛发生长期的小鼠皮肤伤口愈合率显著高于毛发静止期的小鼠伤口愈合率。HE染色显示毛发生长期小鼠伤口周围表皮细胞层较多,且表皮细胞向伤口迁移增强;BrdU检测显示毛发生长期小鼠皮肤伤口周围表皮BrdU+细胞数多于毛发静止期小鼠。结论毛发生长期的小鼠皮肤创伤愈合率高于毛发静止期小鼠,这一结果为进一步探讨毛囊在创伤愈合过程中的作用提供研究基础,也为选择皮肤创伤愈合动物模型提供指导。

Objective To investigate the skin wound healing speed of C57BL/6 mice at various stages of hair growth cycle. Methods Mouse skin wounding models were established and wound healing rate in the following manner was calculated： Wound closure rate （%） = [（Original wound area - Open area on the final dayi/Original wound area] × 100%. Wound healing speed of mouse at hair anagen stages and at hair telogen stages was compared. H＆E staining was adopted to compare differences in morphological and histological characteristics of wound healing. BrdU was used to detect cell proliferation around wounds. Results Wound healing rate of mouse at hair anagen stages was significant higher than that of mouse at telogen stages. H＆E staining showed that mice presented relatively more epidermal cell layers around wounds and epidermal cell layers migrated faster at hair anagen stages. BrdU detection showed that mice at hair anagen stages had a larger number of BrdU＋ epidermal cells around wounds than mouse at telogen stages. Conclusion Mice at hair anagen stages enjoy a faster skin wound healing rate than mice at telogen stages, which provides a basis for further research into the role of hair follicle in wound healing process as well as a guidance on how to choose animal models for study of skin wound healing.