LIM矿化蛋白1过表达对骨肉瘤细胞系OS-9901细胞增殖的影响

The effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation

目的探讨骨肉瘤细胞系OS-9901细胞中LIM矿化蛋白1(LMP-1)过表达对该细胞增殖的影响。方法构建LMP-1过表达慢病毒载体,通过脂质体转染进骨肉瘤细胞系OS-9901细胞。通过荧光定量PCR和Western blot检测LMP-1过表达情况,并通过BrdU掺入实验比较LMP-1过表达前后细胞增殖情况。结果转染了LMP-1过表达慢病毒载体6 h后细胞内LMP-1 mRNA和蛋白表达均无显著变化(P>0.05),且DNA合成量无显著变化。而当LMP-1过表达慢病毒载体转染细胞12、24和48 h后细胞内LMP-1 mRNA和蛋白表达量均显著提高的同时,DNA合成量显著降低(P<0.05)。结论 LMP-1过表达可抑制骨肉瘤细胞系OS-9901细胞增殖。

Objective To investigate the effects of LIM mineralization protein 1 over-expression on the osteosarcoma cell line OS-9901 proliferation. Methods LIM mineralization protein overexpression lentiviral vector was constructed and transfected into osteosarcoma cell line OS-9901 cells by liposome. Quantitative PCR and Western blot were performed to measure the relative mRNA and protein expression of LIM mineralization protein 1, and BrdU assay was performed to detect cell proliferation before and after the LMP-1 over-expression. Results LMP-1 mRNA and protein expression did not change significantly （P〉0.05） at 6 h after cells transfected with the LMP-1 expression lentiviral vector. When LMP-1 mRNA and protein expression were significantly increased at 12h, 24h and 48h after cells transfected with the LMP-1 expression lentiviral vector, DNA synthesis was significantly lower （P〈0.05）. Conclusion LIM mineralization protein 1 overexpression can inhibit osteosarcoma cell line OS-9901 cell proliferation.