不同状态巨噬细胞对RSC96细胞NGF及Laminin表达的影响

The effects of macrophage in different activation states on NGF and Laminin expressions of RSC96 cells

目的研究不同活化状态的巨噬细胞与雪旺细胞株(RSC96)共培养条件下雪旺细胞神经营养因子(NGF)及层粘连蛋白(Laminin,LN)的表达情况。方法利用Transwell建立大鼠腹膜腔巨噬细胞和RSC96细胞的共培养体系,分别将RSC96细胞与未激活巨噬细胞、激活态巨噬细胞共培养;采用Real-time PCR检测、Western blot分析、以及酶联ELISA测定等研究手段,比较各组NGF及LN的表达差异。结果与未激活巨噬细胞共培养组RSC96细胞NGF表达增强,激活态组表达明显增强;而各组LN表达差异不明显。结论巨噬细胞对RSC96细胞NGF表达有促进作用,受其活化状态的影响,激活状态的巨噬细胞的促进作用更明显;对影响RSC96细胞迁移功能的LN的表达作用不明显,无统计学意义,有待进一步研究。

Objective To study the expression of NGF and Laminin in RSC96 cells under co-culture with macrophages in different activation states. Methods A Transwell system was built to co-culture RSC96 cells with rat peritoneal macrophages. Real-time PCR, western blotting, and ELISA were used to detect NGF and LN expression in each group. R0sults Under macrophage co-culture, the NGF expression in RSC96 cells was increased. A significant promotion has been found with activated macrophages. With regard to LN expression, no significant difference was detected. Conclusions The macrophage could contribute to the NGF expression in RSC96 cells, the effect of which is influenced by the activation states. Activated macrophages showed greater effect. No such promotion is found on LN expression, which is associated more with the migration of the Schwann cells. Further research is needed.