四种固定液对肾上腺组织冰冻切片固定效果的比较

Comparison of fixation effects of frozen section of adrenal glands fixed by four fixatives

目的以肾上腺组织为例观察比较丙酮、4%多聚甲醛、AAF和95%乙醇四种固定液的固定效果,为临床做出更好的快速冰冻切片,及时、准确的做出病理诊断提供实验基础。方法取人正常肾上腺组织,冰冻切片后分别用四种固定液进行固定,然后行HE染色,观察各组的组织结构、细胞核染色、细胞质染色和核质对比度情况。结果经冷丙酮固定的肾上腺组织切片,镜下组织结构清晰,高倍镜下观察:细胞核形态清晰完整,核染色和胞质染色清晰对比度好;经4%多聚甲醛固定的切片,镜下组织结构不清,细胞核形态较清晰,核染色和胞质染色对比度差;经AAF液固定的组织切片,镜下组织结构尚清晰,细胞核部分溶解,核染色均一,胞质染色较清晰;经95%乙醇固定的组织,组织结构尚清晰,核溶解普遍,胞质染色不清晰。结论四种固定液都可用于固定肾上腺组织及其它细胞成分较多的病理肿瘤组织,但冷丙酮效果最好。

Objective Comparing the fixation effects of four fixatives,acetone ,4% formaldehyde, AAF and 95% alcohol for adrenal glands, so as to provide experimental proof for making rapid and high quality frozen section clinically. Methods Human normal adrenal glands were subjected to the technique of frozen section and then followed by different fixation and H.E. staining. The slructures of all H.E. stained frozen sections of adrenal glands were examined carefully. Results Specimens fixed by acetone showed good effect with no structure contraction. The group fixed by 4% formaldehyde exhibited unclear structures observed by low magnification. The group fixed by AAF exhibited both clear structures and good staining effects ,however, partial nucleus shrinkage was observed. Specimens fixed by 95% alcohol showed unclear nuclear structure generally. Conclusions These four fixatives could be used for all swelling tissue similar to adrenal glands with the standing out as best fixative.