脚桥被盖核深部电刺激对帕金森病模型大鼠步态的改善作用

Deep brain stimulation of the pedunculopontine tegmental nucleus improves Parkinsonian rats＇gait

目的应用Catwalk自动步态分析系统评估脚桥被盖核（PPTg）深部电刺激（DBS）对偏侧帕金森病（PD、模型大鼠的步态影响。方法运用立体定向术在15只SD雄性大鼠的右侧前脑内侧纵束（MFBl注入6-羟多巴胺（6．OHDA）制成偏侧PD模型大鼠，同时将刺激电极植入同侧PPTg。术后4周，PPTg—DBS前后分别进行Catwalk自动步态分析检测。结果与PPTg—DBS前比较，偏侧PD模型大鼠在PPTg—DBS后，4只脚爪的步幅、摆动速度明显提高，支撑相时间、最大接触面积时间比率明显降低；患侧的前后脚爪的最大接触面积和平均压力明显升高，差异均有统计学意义（P〈0．05）。结论PPTg—DBS可有效改善偏侧PD模型大鼠的步态，Catwalk自动步态分析系统可客观、有效评估大鼠步态改变。

Objective To access the effect deep brain stimulation （DBS） in the pedunculopontine tegmental nucleus （PPTg） on gait of Parkinson disease （PD） rat models using Catwalk automated gait analysis system. Methods Fifteen male Sprague Dawley rats received stereotactic injection of 6-hydroxydopamine （6-OHDA） in the right medial forebrain bundle （MFB） to be unilateral PD rat models and simultaneously received an electrode implantion into the ipsilateral PPTg. Catwalk automated gait analysis system was employed to investigate the changes in gait parameters before and four weeks after surgery. Results After the PPTg-DBS, the stride and swing speeds were significantly increased and the stance and max contact （%） were significantly decreased in all four paws; the max contact area and mean intensity significantly decreased in the affected paw. Conclusion The PPTg-DBS can effectively improve gait function of unilateral PD rat models, and catwalk automated gait analysis system can objectively and effectively evaluate the gait changes.