蛋白激酶C在偏头痛中枢敏感化形成过程中的作用研究

Role of protein kinase C in the mechanism of central sensitization of migraine

目的探讨蛋白激酶c（PKc）在偏头痛中枢敏感化形成过程中的作用。方法60只SD大鼠按随机数字表法分为正常对照组、假手术组、偏头痛模型组、氯仿组和PKC抑制剂H-7组，每组各12只。偏头痛模型组、氯仿组和H-7组大鼠采用Moskowitz法建立硬脑膜神经源性炎症偏头痛模型，并相应侧脑室注射给药。应用激光多普勒血流量图像仪、多导电生理记录仪分别对每组大鼠进行硬脑膜血流量监测和三叉神经脊束核神经元放电记录，分析比较硬脑膜血流量和神经元放电频率的变化。结果造模后2h偏头痛模型组与假手术组相比硬脑膜血流量明显增加，差异有统计学意义（P〈0．05）：H-7组与偏头痛模型组相比硬脑膜血流量明显减少，差异有统计学意义（P〈0．05）；H-7组与假手术组相比硬脑膜血流量明显减少，差异有统计学意义（P〈0．05）。偏头痛大鼠模型复制后，三叉神经脊束核细胞外放电频率增加，造模后2h为造模前的323．82％±11．00％：H-7组与偏头痛模型组相比放电频率明显下降，差异有统计学意义（P〈O．05）；H-7组与假手术组相比放电频率无明显变化。结论PKC可能参与偏头痛中枢敏感化的形成。

Objective To explore the role of protein kinase C （PKC） in the mechanism of central sensitization of migraine. Methods Sixty healthy adult male SD rats, weighting from 200 to 250 g, were randomly divided into five groups： normal group, sham-operated group, migraine model group, chloroform treatment group and H-7 （the inhibitor of PKC） treatment group （n=12）. Dural blood flow monitor was performed by laser Doppler blood flow imager and extracellular discharge frequency in the spinal trigeminal nucleus was observed by multi-conductive polygraph; the dural blood flow and discharge frequency changes were analyzed and compared. Results Two hours after the success of model making, the dural blood flow in the migraine model group increased obviously as compared with that in the sham-operated group （P〈0.05）; as compared with that in the migraine model group, the dural blood flow in the H-7 treatment group decreased obviously （P〈0.05）; as compared with sham operation group, blood flow decreased obviously in H-7 group （P〈0.05）. Extracellular discharge frequency in the spinal trigeminal nucleus increased 2 h after the success of model making; 2 hours after model making, the extracellular discharge frequency was （323.82±11.00） % of baseline level; as compared with that in the migraine model group, discharge frequency in the H-7 treatment group decreased obviously （P〈0.05）; as compared with that in the sham-operated group, discharge frequency in the H-7 treatment group had no obvious changes （P〉 0.05）. Conclusion PKC may play an important role in the mechanism of central sensitization of migraine.