摘要
目的观察体外培养过程中胎儿软骨终板细胞Ⅱ型胶原和蛋白多糖变化的相关性。方法采用含体积分数10%胎牛血清的低糖DMEM培养基培养胎儿软骨终板细胞,应用Western blot和RT-PCR法检测第1代到第8代胎儿软骨终板细胞Ⅱ型胶原和蛋白多糖及其mRNA的表达情况,直线回归分析Ⅱ型胶原和蛋白多糖及其mRNA表达的相关性。结果第1～8代培养细胞的Ⅱ型胶原及蛋白多糖mRNA及蛋白表达水平两两比较差异均有统计学意义(P<0.05);Ⅱ型胶原及其mRNA表达与蛋白多糖及其mRNA的表达呈明显正相关(r=0.994 7,P=0.014;r=0.945 3,P=0.017)。结论抑制Ⅱ型胶原或蛋白多糖中一种物质下降可同时阻止另一种物质下降,从而延缓椎间盘退变发生。
Objective To observe the correlation of collagenⅡwith proteoglycan of fetal cartilage endplate cells in the culturing process in vitro. Methods Fetal cartilage endplate cells were cultured by using L-DMEM medium containing volume percentage of 10% fetal bovine serum. The fetal cartilage endplate cells collagen Ⅱ, proteoglycan and theirs mRNA from the 1st to the 8th generation were detected by Western blot and RT-PCR technique. The correlation of collagen Ⅱ and proteoglycan with their mRNA expression was analyzed by linear regression. Results There were significant differences between collagen Ⅱ and its mRNA expression, as well as between proteoglycan and its mRNA expression of fetal cartilage endplate cells from the 1st to the 8th generation（P〈0.05）. Collagen Ⅱ and its mRNA expression were positively correlated with proteoglycan and its mRNA expression （r=0. 994 7, P=0. 014; r=0. 945 3, P=0. 017）. Conclusion To restrain the decline of one of collagen Ⅱ and proteoglycan can prevent the other from decreasing in order to delay the degeneration of intervertebral disc.
出处
《中华实用诊断与治疗杂志》
2013年第6期560-561,564,共3页
Journal of Chinese Practical Diagnosis and Therapy
