平菇病毒dsRNA的提取及脱除

Extraction and Elimination of Virus of dsRNA from Pleurotus ostreatus

以平菇为试材,采用改进SDS法从平菇组织中提取病毒特异dsRNA,并分别采用化学药剂-菌丝尖端处理和高温-菌丝尖端处理对平菇中的病毒进行了脱毒处理。结果表明:病毒dsRNA特点显示其与国外报道的平菇病毒dsRNA一致。其中高温-菌丝尖端脱除处理的效果最好,可有效脱除平菇病毒;病毒唑、放线菌酮及病毒唑与盐酸吗啉胍联合菌丝尖端脱除处理,可使病毒dsRNA条带减弱。该试验建立了从平菇组织中提取病毒dsRNA的技术体系,可有效对平菇中的病毒进行鉴定。

Taking Pleurotus ostreatus as material, the special dsRNAs of virus were extracted from Pleurotus ostreatus with modified SDS method,and elimination of virus in Pleurotus ostreatus was performed with reagent-mycelial tip-cutting and high temperature-mycelial tip-cutting methods. The results showed that the dsRNA was the same as one in foreign studies. The virus could be effectively eliminated with reagent-mycelial tip-cutting method, and the methods, including of ribavirin-mycelial tip-cutting, cycloheximide-mycelial tip-cutting, ribavirin and moroxydine hydrochloride-mycelial tip-cutting, could reduce the quantity of dsRNA of virus. The system of extraction of dsRNA was developed using tissues of Pleurotus ostreatus as material. In addition,the technique could effectively detect virus in Pleurotus ostreatus.