HPLC法同时测定茵栀黄分散片中绿原酸与栀子苷的含量

Simultaneous Determination Chlorogenic Acid and Jasminoidin of Yinzhihuang Dispersible Tablets by HPLC

目的:建立同时测定茵栀黄分散片中绿原酸与栀子苷的含量测定方法。方法:采用HPLC法,Agilent ZorbaxSB-C18(色谱柱(250mm×4.6mm,5μm),流动相:乙腈-0.3%甲酸水,梯度洗脱,柱温30℃,流速:1.0mL.min-1,检测波长:0～9min为327nm(绿原酸),9～30min为238nm(栀子苷)。结果:绿原酸和栀子苷的线性范围分别为1.25～12.50μg.mL-1(r=0.999 6,n=6)、2.23～22.30μg.mL-1(r=0.999 8,n=6),平均回收率分别为99.82%(RSD为1.16%,n=6)与98.49%(RSD为2.27%,n=6)。结论:该方法简便、准确、重现性好,可用于茵栀黄分散片中绿原酸与栀子苷含量的同时测定。

Objective ： To develop a method for determining chlorogenic acid and jasminoidin in Yinzhihuang dispersible tablets simultaneously by HPLC. Methods： HPLC method was developed for the determinition. Agilent ZorbaxSB-C18 （250mm×4.6mm,5μm） was used. mobile phase was composed acetonitrile-0.3% formic acid water solution with linear gradient elution, The column temperature was 30℃, the flow rate was 1.0 mL·min^-1 , the wavelength was acquired at 327 nm from 0 to 9 min for chlorogenic acid, 238 nm from 9 to 30 min for jasminoidin. Results：The linear range of chlorogenie acid and jasminoidin were 1.25- 12.50 μg · mL^-1（r=0.999 6, n=6）, 2.23 - 22.30 μg · mL^-1（r=0.999 8, n=6）. The avergerecoverywere99.82%（RSD=1.16%,n=6） and 98.49 % （RSD= 2.27 %, n= 6）. Conclusion：The method is simple, reliable, accurate and can be applied to the determination of chlorogenic acid and jasminoidin in Yinzhihuang dispersible tablets simultaneously.