摘要
目的:建立浙贝母的HPLC-ELSD含量测定方法,完善椰露止咳合剂的质量标准。方法:采用HPLC-ELSD法测定贝母素甲、贝母素乙的含量。色谱柱:Agilent ZORBAX SB-C18柱;流动相:乙睛-0.1%二乙胺溶液(70∶30);流速:1.0mL/min;检测器:蒸发光散射检测器。结果:贝母素甲在0.101～2.513g范围内呈良好线性关系(r=0.999 4),平均加样回收率为98.8%(n=9);贝母素乙在0.050～1.998g范围内呈良好线性关系(r=0.999 0),平均加样回收率为97.6%(n=9)。结论:该方法操作简单、重现性好、结果准确可靠,可用于椰露止咳合剂的质量控制。
Objective ： Methods ： To study the quality standard of Yeluzhike Mixture and establish a HPLC-ELSD method for determining Bulbus Fritillariae Thunbergii. Results ： HPLC-ELSD method was established for the determination of Peimine and Peiminine. The Agilent ZORBAX SB-C18 column was used with the evaporative light scattering detector for detection, acetonitrile-0.1 % diethyb amine （70 ： 30） as the mobile phase and the flow rate of 1.0mL/min. Peimine in 0. 101-2. 513g within the scope of a good linear relationship （r= 0. 9994）, the average recovery rate was 98.8 % （n= 9） ; Peiminine in 0. 050- 1. 998g range showed a good linear relationship（r = 0.9990）, the average recovery rate was 97.6 % （n = 9 ）. Conclusion ： The method was convenient, reproducible, relia- ble, and suitable for quality control of Yeluzhike Mixture.
出处
《亚太传统医药》
2013年第6期57-59,共3页
Asia-Pacific Traditional Medicine
