子宫内膜息肉及子宫内膜癌组织中雌激素受体β的甲基化与表达研究

Research for methylation of estrogen receptor β and expression of ER-β in endometrial polyps and endometrial carcer

目的检测正常子宫内膜、子宫内膜息肉及子宫内膜癌中雌激素受体β(estrogen receptor-β,ER-β)基因启动子区CpG岛的甲基化状态及ER-β蛋白的表达,同时分析ER-β启动子甲基化与ER-β的表达间的关系,以探讨子宫内膜息肉的发病机制。方法采用免疫组化ELIVISONTM二步法检测40例正常子宫内膜、40例子宫内膜息肉及26例子宫内膜癌组织中ER-β蛋白的表达,同时运用甲基化特异性PCR(MSP)检测上述组织中ER-β的甲基化情况。结果正常子宫内膜中ER-β的表达较子宫内膜息肉及子宫内膜癌显著增高(均P<0.01),子宫内膜息肉与子宫内膜癌则无显著差异(P>0.05)。正常子宫内膜及子宫内膜息肉组织中ER-β基因甲基化率较子宫内膜癌显著降低(均P<0.05)。正常子宫内膜、子宫内膜息肉及子宫内膜癌组织中ER-β的阳性表达与ER-β基因启动子甲基化状态均呈负相关。结论子宫内膜组织中ER-β的基因甲基化可能与子宫内膜息肉乃至子宫内膜癌的发生和复发密切相关。

Objective To detect methylaion status of CpG islands of estrogen receptor-β （ER-β） promoter and ER-β expression in normal endometrium, endometrial polyps and endometrial cancer. Methods We detected ER βprotein expression in tissues using two-step immunohistochemical ELIVISONTM. Methylation specific PCR （MSP） were used to detected methylation of ER-β. Results ER-β protein was significantly increased in normal endometrial tissue（P 〈0.01 ）. There was no difference of ER protein between endometrial polyps and endometrial cancer tissue（ P 〉 0.05 ）. Methylation level of ER-β gene were significantly decreased in the normal endometriumr and endometrial polyp compared with that of endometrial cancer（ P 〈 0.05 ）. The relative of ER-β and ER-β gene promoter methylation was negative in tissues as above. Conclusions ER-β gene methylation in endometrial tissue may promote endometrial polyps and cndometrial cancer recurrence and further development.