磷脂酰肌醇蛋白聚糖-3在原发性肝癌中的表达及临床意义

Expression and clinical significance of glypican-3 in primary hepatocellular carcinoma

目的探讨磷脂酰肌醇蛋白聚糖-3(glypican-3,GPC3)在原发性肝癌中的表达及临床意义。方法采用免疫组化法检测45例手术切除的原发性肝癌及癌旁组织中GPC3的表达水平,比较原发性肝癌不同临床病理特征中GPC3的表达差异,分析GPC3表达与临床和病理因素的相关性。结果肝癌组织GPC3表达评分[(10.3±2.5)分]显著高于癌旁组织[(3.2±0.8)分],差异有统计学意义(P<0.05);不同性别、年龄组间GPC3蛋白表达比较,差异无统计学意义(P>0.05),而不同临床分期、肿瘤直径及有无转移患者比较,差异有统计学意义,GPC3在临床分期晚、肿瘤直径大、分化程度低及远处有转移的患者肝癌组织中表达强度增高(P<0.05)。logistic回归分析显示,PHC患者肝癌组织中GPC3表达与临床分期、肿瘤直径、分化程度及远处有转移等因素紧切相关(OR=0.623～0.960,P<0.05)。结论 GPC3蛋白在肝癌组织中呈高表达状态,对于其病情的诊断及预后评估具有重要价值。

Objective To study the expression and clinical significance of glypican-3 （GPC3） in primary hepatic earcinoma（PHC）. Methods Immunohistochemistry was applied to detect GPC3 expression in 45 cases of PHC. The corre- lation between exprssion levels of GPC3 and clinieopathologic factors was analyzed. Results The exprssion levels of GPC3 in PHC tissues were significantly higher than those in adjacent normal tissues [（10.3~2.5）vs.（3.2＋0.8）, P 〈 0.05]. There were no significant differences of GPC3 expression levels in different age group , in male and female groups （P 〉 0.05）. But there were significant differences in different clinical stage, tumor diameter, tumor cell differentiation and metastasis or not. The expression degree of GPC3 highly increased in late clinical stage, larger tumor size, less differ- entiation and metastasis. The expression levels of GPC3 in hepatocellular carcinoma tissues were closely related to clinical stage, tumor size, differentiation and metastasis （OR = 0.623-0.960, P 〈 0.05）. Conclusion GPC3 expresses high in PHC tissues, which could act as a biomarker for evaluating disease condition and prognosis.