长链非编码RNA MEG3在胚胎性横纹肌肉瘤凋亡中的研究

Apoptotic study of long non-coding RNA MEG3 in embryonal rhabdomyosarcoma

目的研究人类长链非编码RNA(lncRNAs)母系印记基因3(MEG3)在胚胎性横纹肌肉瘤凋亡中的作用,并探讨其分子机制。方法分析18例胚胎性横纹肌肉瘤的临床特征,免疫组织化学法检测肿瘤组织凋亡相关基因产物的表达,TUNEL法检测凋亡指数,qRT-PCR法检测MEG3在18例胚胎性横纹肌肉瘤及7例正常骨骼肌中的表达情况。结果 18例胚胎性横纹肌肉瘤临床表现因发病部位不同可有多种临床症状,免疫组织化学检测与肿瘤凋亡相关的P53、Caspase-9及Caspase-3均阳性表达,Ki-67核增殖指数达40%;TUNEL法检测平均凋亡指数为37%;qRT-PCR法检测18例胚胎性横纹肌肉瘤组织中MEG3表达较7例正常骨骼肌组织显著增高(P<0.05)。结论 MEG3与胚胎性横纹肌肉瘤凋亡呈正相关关系。

Objective To study the role of human long non-coding RNAs （｜ncRNAs） maternally imprinted genes 3 （MEG3） on the apoptosis of embryonal rhabdomyosarcoma （eRMS） as well as its molecular mechanisms. Methods The clinical manifestations, immunohistochemical staining and TUNEL apoptotic experiment were observed in 18 eRMS samples and 7 normal skeletal muscle samples. Real-time quantitative polymerase chain reaction （qRT-PCR） was used to analyze the expression of MEG3. Results Ten males and 8 females aged from 4 months to 6 years （ with an average age of 2. 5 years） were enrolled in this study, eRMS usually showed diverse clinical symptoms according to its anatomic origins, which is generally due to the obstruction of the mass. Immunohistochemistry indicated positive expression of P53, Caspase-9 and Caspase-3. The proliferation index of Ki-67 was more than 40%. Tunel apoptotic assay showed yellow-green fluorescence in tumor cells, qRT-PCR analysis indicated an over expression of MEG3 in eRMS samples （P 〈 0.05 ）. Conclusions MEG3 had a positive correlation with the apoptosis in eRMS.