鸡传染性鼻炎灭活疫苗生产检验用副鸡禽杆菌Hpg-8株的保存期试验

Preservation Period Test of the Hpg-8 Strain of A.paragallinarum Master Seed of Infectious Croyza Inactivated Vaccine

对不同冻干年代的副鸡禽杆菌Hpg-8株菌种分别进行了毒力测定和免疫原性试验。分别用1991、1997、2001、2011年冻干的菌种,以约3万CFU/羽眶下窦接种2月龄SPF母鸡,结果均可使鸡5/5发病;分别用1991、1997、2011年冻干的菌种,经繁殖制备疫苗后接种SPF鸡,结果可提供8/8、7/7、7/7保护。结果表明,副鸡禽杆菌Hpg-8株在-70℃保存10、14、20年后的毒力和在-70℃保存14、20年后的免疫原性仍符合《中华人民共和国兽用生物制品规程》(二○○○版)规定。

The virulence on SPF chicken and immunogenicity of the Hpg - 8 strain cultures of A. paragallinarum manufactured at different years were tested respectively. 5/5 chicken showed the clinical symptoms of infectious coryza after intrasinus injected respectively with about 30000 CFU of the Hpg - 8 strain cultures of A. paragallinarum producted at 1991, 1997, 2001 and 2011. The protection rates of 8/8, 7/7 and 7/7 were obtained after chickens were inoculated with the coryza vaccine with the Hpg -8 strain cultures of A. paragallinarum producted at 1991, 1997, and 2011. The results indicated that the virulence on SPF chicken of the Hpg-8 strain cultures of A. paragallinarum stored at -70 ℃ for 10, 14, 20 years and the immunogenicity of the Hpg-8 strain cultures of A. paragallinarum stored at -70 ℃ for 14, 20 years still conformed to the regulations of The People＇ s Republic of China Veterinary Biological Products （2000 edition）.