2型糖尿病大鼠心血管风险相关生物标志物的变化研究

Variation of cardiovascular risk biomarkers in type 2 diabetes mellitus

以小剂量链脲佐菌素(STZ)诱导的2型糖尿病大鼠为实验模型,研究2型糖尿病大鼠糖代谢、脂代谢及心血管风险相关生物标志物的变化趋势和相互关系。将实验动物分为空白对照组、高脂对照组和2型糖尿病组,分别于注射STZ造模前和造模后第2、4、7、9周空腹取血,测定血液中糖、脂代谢生物标志物、不对称二甲基精氨酸(ADMA)、总同型半胱氨酸(tHcy)、脂联素(APN)和正五聚蛋白3(PTX3)浓度。结果表明,2型糖尿病组空腹血糖、胰岛素抵抗指数、甘油三酯、总胆固醇、低密度脂蛋白胆固醇显著高于空白对照组和高脂对照组。与空白对照组相比,2型糖尿病组ADMA逐渐升高,tHcy逐渐降低,APN显著降低,PTX3显著升高。2型糖尿病大鼠与心血管风险相关的生物标志物ADMA、tHcy、APN、PTX3与糖、脂代谢生物标志物之间存在密切的相关性,在评价其心血管风险时应同时监测上述多个生物标志物。

The aim of study was to determine the relationship between the biomarker of glucose and lipid metabo- lism and NG, NG＇-asymmetric dimethylarginine （ADMA）, total homocysteine （tHcy）, adiponectin （APN） and pentraxin 3 （PTX3） in type 2 diabetes mellitus rats. Diabetes was induced in Sprague-Dawley rats by a single intraperitoneal injection of 35mg/kg streptozotocin （STZ）. Before and 2nd, 4th, 7th, 9th weeks after STZ injection, fasting plasma glucose （FPG）, fasting insulin APN, PTX3 were determined. Compared with （FINS）, OGTF, serum lipid （ TC, TG and LDL-C）, ADMA, tHcy, the groups of control and high fat diet （HFD）, FPG, Homeostasis model assessment for insulin resistence index （ HOMA-IR）, TC, TG and LDL-C level of type 2 diabetes mellitus （T2DM） groups were significantly increased. In T2DM group, the concentration of ADMA increased gradually, tHcy decreased by degrees, APN reduced significantly, the PTX3 elevated remarkably. Our results indicate that ADMA, tHcy, APN, PTX3 are well correlated with glucose and lipid metabolism biomarkers. The plurality of biomarkers mentioned before should be monitored to evaluate the cardiovascular risk of type 2 diabetes mellitus.