液相色谱质谱-质谱法检测小麦叶片中茉莉酸的研究

A study on the detection of JAs in wheat leaves by liquid chromatography mass-mass spectrometer

为研究小麦茉莉酸抗病信号途径,建立了一种小麦(Triticum aestivum L.)抗病反应过程中叶片内源激素茉莉酸(jasmonic acid,JA)含量测定的方法.以"中国春"和"09X15"小麦幼苗为材料,在接种小麦白粉病菌(Blume-ria graminis f.sp.tritici)后,用优化的样品前处理方法提取叶片内源JA,在液相色谱质谱联用仪上,按照优化的高效液相色谱质谱-质谱条件,测定小麦白粉病菌侵染后不同时间小麦幼苗叶片内JA含量.结果表明,建立的小麦叶片JA含量液相色谱质谱-质谱测定方法加标回收率达到97%,最低检测限(LOD)为1μg.L-1,能满足小麦抗病反应中叶片JA含量变化测定要求.

In order to study the function of jasmonic acid （JA） in wheat leaves during powdery mildew resistant reaction, a method has been established for determining concentration of endogenous jasmonic acid （JA） in wheat leaves. The JA in Chinese Spring and 09X15 seedling leaves after inoculation with Blumeria graminis f. sp, tritici was extracted by optimized protocol and measured by parameter opti- mized Liquid Chromatography Mass-Mass Spectrometer. The results showed that the recovery rate of added standard MeJA reached 97% and the lowest detection limit （LOD） was 1μg· L^-1. This method can meet the requirement of jasmonates （JAs） measurement in wheat leaves during disease resistance.