组蛋白H3赖氨酸27和36位甲基化修饰的鉴定

Identification of methylation at lysine 27 and 36 sites in histone H3

采用液相色谱-质谱联用技术结合生物信息学分析手段,研究Hela细胞组蛋白H3赖氨酸(Lysine(K))K27和K36位点带有甲基化和二甲基化修饰的多肽鉴定,通过二级质谱碎片解析和二级碎片丰度分析,对组蛋白H3赖氨酸K27和K36上甲基化和二甲基化修饰进行了鉴定和分析。

we combined high performance liquid chromatography and tandem mass spectrometry（HPLC-MS/MS） with bioinformatics tools to analyze the isobaric modified peptides which were methylated and dimethylated at either lysine（K） 27 or/and K36 from histone H3.They were identified and dissected through alignment of every fragment ion,and the two modified sites were further analyzed according to their relative intensities of MS/MS spectra.