基于巯基-烯点击反应制备有机-无机杂化硼酸亲和整体柱用于糖蛋白的选择性富集

Preparation of organic-inorganic hybrid boronate affinity monolith via thiol-ene click reaction for specific capture of glycoproteins

发展了以巯基-烯点击反应制备有机-无机杂化硼酸亲和整体柱的新方法。首先以四甲氧基硅烷(TMOS)和巯丙基三甲氧基硅烷(MPTMS)作为反应单体,采用溶胶-凝胶反应制备表面含巯基的硅胶整体柱。然后利用巯基-烯(thiol-ene)的点击反应在整体柱上修饰硼酸配基3-丙烯酰胺基苯硼酸(AAPBA),制成AAPBA-硅胶杂化亲和整体柱。对影响硼酸亲和整体柱性能的条件如TMOS与MPTMS的比例、聚乙二醇和甲醇的用量等进行了优化。并采用扫描电镜、红外光谱等分析仪器对整体柱形貌和机械稳定性能进行了表征。研究了AAPBA-硅胶杂化亲和整体柱的分离性能,结果表明,其在中性条件下对含有顺式二醇的生物小分子核苷具有良好的特异亲和能力,并已成功地应用于卵清蛋白、辣根过氧化物酶等糖蛋白的分离。基于巯基-烯反应的制备方法新颖、可靠,可用于制备多种不同类型的硼酸亲和整体柱,具有较大的应用前景。

A novel strategy for the preparation of the organic-inorganic hybrid boronate affinity monolith was developed via the ＂thiol-ene＂ click reaction.A thiol group-modified silica monolith was first synthesized via the sol-gel process by the in situ co-condensation with tetramethoxysilane（TMOS） and 3-mercaptopropyltrimethoxysilane（MPTMS） as precursors.Then 3-acrylamidophenylboronic acid（AAPBA） was covalently immobilized on the hybrid monolith via the ＂thiol-ene＂ click reaction to form AAPBA-silica hybrid affinity monolith.The reaction conditions for the preparation of AAPBA-silica hybrid affinity monolith were optimized,including the ratio of TMOS to MPTMS,the contents of poly（ethylene glycol）（PEG） and methanol.The morphology and mechanical stability of the boronate affinity monolith were characterized and evaluated by scanning electron microscopy and Fourier-transform infrared spectroscopy.The obtained boronate affinity hybrid monolith exhibited excellent specificity toward the nucleosides containing cis-diols under neutral conditions.It was further applied to the specific capture of the glycoproteins ovalbumin and horseradish peroxidase.The method is novel and reliable,which has a great potential for the preparation of different kinds of the boronate affinity monoliths.