络合萃取法提取发酵液中丁二酸铵

Recovery of Succinate from Fermentation Broth Based on Complexation Extraction Process

为提高微生物发酵法生产丁二酸的产品提取收率,用三辛胺/正辛醇体系对发酵液进行络合萃取。比较硫酸和阳离子交换树脂两种前处理发酵液方式对络合萃取丁二酸的影响以及用氨水反萃制备丁二酸铵。结果表明,通过阳离子交换树脂前处理发酵液,三辛胺/正辛醇体系对丁二酸的萃取率高于硫酸前处理,达到91.3%;氨水是一种优良反萃剂;萃取剂重复使用5次,平均萃取率为90.4%,平均反萃率为94.9%,且萃取率和反萃率未见明显下降。

To improve the extraction yield of succinic acid produced by microbial fermentation, trioetylamine/octanol was used as extraction agent to extract succinate from fermentation broth. The pre-treatment of fermentation broth as well as the preparation of suecinic acid ammonium using ammonia in the re-extraction were investigated. The results show that the rate of succinic acid extraction from fermentation broth pre-treated by cation exchange resin reached 91.3% , which was higher than that by sulfuric acid. Ammonia was an excellent re-extraction agent and the extractant （ trioctylamine / octano） could be reused. When the extractant was recycled five times,the average rate of extraction and that of re-extraction were 90.4% and 94.9% ,respectively,showing no obvious decline.