摘要
目的研究急性肺损伤（acute lung injury，ALI）幼鼠肺组织中水通道蛋白-1（aquaporin-1，AQP-1）的表达变化，探讨糖皮质激素治疗ALI的可能机制。方法将实验动物分为生理盐水对照组（对照组）、致ALI组（lipopolysaccharide，LPS组）、甲泼尼龙（memylprednisolone，Mp）治疗组（Mp组）。观察6h后测定动脉血氧分压、肺湿干重比值，并采用免疫组织化学方法和RT—PCR法检测各组大鼠肺组织中AQP-1的表达变化及对肺组织进行常规病理染色观察。结果RT—PCR结果显示LPS组AQP-1mRNA相对表达量与对照组相比明显减少（0．28±0．05vs0．50±0．24，P〈0．05），Mp组AQP-1mRNA相对表达量与LPS组相比明显增加（0．47±0．16vs0．28±0．05，P〈0．05）。免疫组织化学结果与RT，PCR结果一致。同时Mp组血氧明显改善（P〈0．01），肺湿干重比值下降（P〈0．05），肺组织损伤减轻。结论糖皮质激素对ALI的治疗作用可能与其影响AQP-1的表达有关。
Objective To explore the mechanism of glucocorticoids in the treatment of acute lung injury（ALI） by studying the expression changes of aquaporin-1 （ AQP-1 ） in the lung. Methods Sprague- Dawley rats were randomly divided into 3 groups, which were ALI group injected by lipopolysaccharide （ LPS group） ,therapy group treated by methylprednisolone （Nip） after LPS injection （Mp group） and control group injected by normal saline. PaO2 and wet/dry mass ratio were assayed at 6 hours after the injection. Changes of AQP-1 expression in the lungs were analyzed by immunohistochemistry and reverse transcription polymerase chain reaction （RT-PCR）, and pathological examination was performed in each group. Results RT-PCR results showed that the expression of AQP-1 in LPS group was lower than that in control group （ 0. 28 ± 0. 05 vs 0. 50 ± 0. 24, P 〈 0. 05 ）, while the expression of AQP-1 in Mp group was higher than that in LPS group （0. 47 ±0. 16 vs 0. 28 ±0. 05 ,P 〈0. 05）. The immunohistochemistry results were consistent to the RT-PCR results, meanwhile glucocorticoids therapy could improve the blood oxygen level （ P 〈 0. 01 ）, reduce the wet/dry mass ratio （ P 〈 0. 05 ） and relieve the lung injury. Conclusion Glucocorticoids may have beneficial effects on ALl by affecting the expression of AQP-1 in the lungs of rats.
出处
《中国小儿急救医学》
CAS
2013年第3期265-267,共3页
Chinese Pediatric Emergency Medicine
基金
辽宁省教育厅高校科研项目（L2010119）
