苹果锚蛋白基因ANK家族生物信息学鉴定分析

Bioinformatics Analysis of ANK Gene Family in Apple

利用生物信息学方法对苹果ANK基因家族成员及分类鉴定，同时对其染色体定位、系统进化关系及芯片表达特性进行了分析。苹果MdANK家族包含351个基因，根据蛋白结构域差异分为16个类别，ANK．M类型最为庞大，有143个ANK蛋白；苹果的17条染色体均有ANK家族基因分布，其中第2条染色体上分布最多，有36个ANK基因。MdANK编码的蛋白在72—2429个氨基酸范围内，等电点在4．30～11．13之间。芯片分析发现，在苹果果实成熟时期及砧木接穗互作过程中，多数MdANK基因的表达都有不同程度变化。

ANK （ankyrin repeat） containing proteins comprise a large protein family, which play important roles in plant growth, development, and signal transduction, no ANK genes have been reported in apple. In this study, MdANK gene family, gene classification, chromosome location, sequence alignment, conserved structure domains and expression profiles of MdANKs were predicted and analyzed with bioinformatics methods, alignment of amino acid sequences and phylogenetic analysis. The results showed that MdANK family contained 351 genes, which was further divided into 16 types and the largest group is the ANK-M type contains 143 proteins. Phylogenic analysis revealed that MdANK genes in apple could be divided into 15 subfamilies （from A to O）according to the phylogenetic analysis tree by MEGA 5. A total of 112 MdANK genes were mapped to 17 chromosomes, whereas 22 MdANK genes were not mapped to any particular chromosome. For example, Chr 2 encompasses the largest number of 36 MdANK genes, while the lowest number （Chr 6, 7 and 8） contained 6 MdANK genes. MdANK proteins containedfrom 72 to 2 429 amino acids and the isoelectric point is from 4.30 to 11.13. Expression analysis showed that the expression levels of most MdANK genes were altered during the ripening process and rootstock-scion interactions process. Our data will provide very useful information for cloning and functional analysis of members of this gene family in apple.