摘要
目的探讨白细胞介素17(IL-17)单核苷酸多态性(SNPs)及血浆水平与慢性HCV感染的关系。方法选择慢性丙型肝炎(CHC)患者228例,健康体检者81例。TaqMan探针法检测IL-17rs8193036及rs2275913位点基因多态性,酶联免疫吸附法检测血浆IL-17水平,分析CHC患者IL—17等位基因、基因型及血浆水平与健康人群的差异。结果CHC患者组与健康对照组IL-17rs8193036位点C、T等位基因及rs2275913位点A、G等位基因分布比较,P值均〉0.05,差异均无统计学意义。CHC患者组与健康对照组的IL-17rs8193036位点基因型CC(46.49%对比41.98%)、CT(45.61%对比44.44%)、1vr(7.89%对比13.58%)分布比较,P值均〉0.05,差异均无统计学意义;rs2275913位点基因型AA(16.23%对比13.58%)、AG(48.25%对比50.62%)、GG(35.53%对比35.800/0)分布比较,P值均〉0.05,差异均无统计学意义。HCV基因1型与2型感染CHC患者IL-17rs8193036及rs2275913位点等位基因及基因型分布比较,P值均〉0.05,差异均无统计学意义。CHC患者组血浆IL-17水平为(97.67士39.68)pg/ml显著高于健康对照组的(71.60±19.78)pg/ml,两组比较t=2.414,P=0.033,差异有统计学意义。结论IL-17高水平表达在HCV感染及慢性化中发挥重要作用,而IL-17rs8193036及rs2275913位点SNPs可能与HCV易感性及持续感染无明确关系。
Objective To investigate the association of single nucleotide polymorphisms (SNPs) in the interleukin 17 (IL-17) gene and serum protein levels in patients with chronic hepatitis C virus (HCV) infection. Methods A total of 228 patients with chronic HCV infection and 81 healthy controls were enrolled in the study. The frequencies oflL-17 rs8193036 and rs2275913 polymorphisms were detected by the TaqMan SNP genotyping assay. Serum levels of IL-17 protein were detected by ELISA. Pairwise comparisons were made by the Chi-square test, and the significance of between-group diffrences was assessed by the Student's t-test with P 〈 0.05. Results The patients with chronic HCV infection and the healthy controls showed similar frequencies of the rs8193036 C/T allele (g2 = 1.428, P = 0.232) and the rs2275913 A/G allele (2'2 = 0.106, P = 0.744). In addition, the two groups showed similar distribution of the rs8193036 CC (chronic HCV infection: 46.49% vs. healthy controls: 41.98%), CT (45.61% vs. 44.44%) and TT (7.89% vs. 13.58%)genotypes (Z2 = 2.346, P = 0.309), and of the rs2275913 AA (16.23% vs. 13.58%), AG (48.25% vs. 50.62%) and GG (35.53% vs. 35.80%) genotypes (Z2 = 0.340,P = 0.844). Subgroup analysis of chronic HCV infection patients slratified according to HCV genotypes 1 and 2 showed no differences in the distribution of rs8193036 and rs2275913 alleles (X2= 1.127,P = 0.288;X2 = 1.088,P = 0.297) and genotypes X2 = 2.825,P = 0.246;X2 = 0.970, P = 0.616). However, the chronic HCV infection group did show significantly higher levels of serum IL-17 than the controls (97.67 士 39.68 vs. 71.60 士 19.78 pg/ml, t = 2.414,P = 0.033). Conclusion Chronic HCV infection is associated with increased serum IL-17; however, the IL-17 polymorphisms rs8193036 and rs2275913 were not associated with chronic HCV infection susceptibility in this study's Chinese cohort.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2013年第6期425-428,共4页
Chinese Journal of Hepatology
基金
河北省科学技术研究与发展计划项目(10276102D)
关键词
肝炎
丙型
慢性
白细胞介素17
多态性
单核苷酸
疾病易感性
Hepatitis C, chronic
Interleukin 17
Polymorphism, single nucleotide
Diseasesusceptibility