白细胞介素17基因多态性及血浆水平与慢性丙型肝炎病毒感染的关系

Relation of IL-17 polymorphisms and serum levels in patients with chronic HCV infection

目的探讨白细胞介素17（IL-17）单核苷酸多态性（SNPs）及血浆水平与慢性HCV感染的关系。方法选择慢性丙型肝炎（CHC）患者228例，健康体检者81例。TaqMan探针法检测IL-17rs8193036及rs2275913位点基因多态性，酶联免疫吸附法检测血浆IL-17水平，分析CHC患者IL—17等位基因、基因型及血浆水平与健康人群的差异。结果CHC患者组与健康对照组IL-17rs8193036位点C、T等位基因及rs2275913位点A、G等位基因分布比较，P值均〉0．05，差异均无统计学意义。CHC患者组与健康对照组的IL-17rs8193036位点基因型CC（46．49％对比41．98％）、CT（45．61％对比44．44％）、1vr（7．89％对比13．58％）分布比较，P值均〉0．05，差异均无统计学意义；rs2275913位点基因型AA（16．23％对比13．58％）、AG（48．25％对比50．62％）、GG（35．53％对比35．800／0）分布比较，P值均〉0．05，差异均无统计学意义。HCV基因1型与2型感染CHC患者IL-17rs8193036及rs2275913位点等位基因及基因型分布比较，P值均〉0．05，差异均无统计学意义。CHC患者组血浆IL-17水平为（97．67士39．68）pg／ml显著高于健康对照组的（71．60±19．78）pg／ml，两组比较t=2．414，P=0．033，差异有统计学意义。结论IL-17高水平表达在HCV感染及慢性化中发挥重要作用，而IL-17rs8193036及rs2275913位点SNPs可能与HCV易感性及持续感染无明确关系。

Objective To investigate the association of single nucleotide polymorphisms （SNPs） in the interleukin 17 （IL-17） gene and serum protein levels in patients with chronic hepatitis C virus （HCV） infection. Methods A total of 228 patients with chronic HCV infection and 81 healthy controls were enrolled in the study. The frequencies oflL-17 rs8193036 and rs2275913 polymorphisms were detected by the TaqMan SNP genotyping assay. Serum levels of IL-17 protein were detected by ELISA. Pairwise comparisons were made by the Chi-square test, and the significance of between-group diffrences was assessed by the Student＇s t-test with P 〈 0.05. Results The patients with chronic HCV infection and the healthy controls showed similar frequencies of the rs8193036 C/T allele （g2 = 1.428, P = 0.232） and the rs2275913 A/G allele （2＇2 = 0.106, P = 0.744）. In addition, the two groups showed similar distribution of the rs8193036 CC （chronic HCV infection： 46.49% vs. healthy controls： 41.98%）, CT （45.61% vs. 44.44%） and TT （7.89% vs. 13.58%）genotypes （Z2 = 2.346, P = 0.309）, and of the rs2275913 AA （16.23% vs. 13.58%）, AG （48.25% vs. 50.62%） and GG （35.53% vs. 35.80%） genotypes （Z2 = 0.340,P = 0.844）. Subgroup analysis of chronic HCV infection patients slratified according to HCV genotypes 1 and 2 showed no differences in the distribution of rs8193036 and rs2275913 alleles （X2= 1.127,P = 0.288;X2 = 1.088,P = 0.297） and genotypes X2 = 2.825,P = 0.246;X2 = 0.970, P = 0.616）. However, the chronic HCV infection group did show significantly higher levels of serum IL-17 than the controls （97.67 士 39.68 vs. 71.60 士 19.78 pg/ml, t = 2.414,P = 0.033）. Conclusion Chronic HCV infection is associated with increased serum IL-17; however, the IL-17 polymorphisms rs8193036 and rs2275913 were not associated with chronic HCV infection susceptibility in this study＇s Chinese cohort.