期刊文献+

蛋白酶体抑制剂MG132减轻大鼠移植胰腺缺血再灌注损伤的作用 被引量:1

Protective effect of MG132 on ischemia-reperfusion injury after pancreaticoduodenal transplantation in rats
原文传递
导出
摘要 目的研究蛋白酶体抑制剂MG132减轻大鼠移植胰腺缺血再灌注(IR)损伤的作用及其机制。方法采用随机数字表法将SD大鼠分为假手术组、IR组和MG132组,IR组和MG132组建立同系大鼠胰腺十二指肠移植模型。分别于再灌注后1、3、6h,检测受鼠血清淀粉酶含量,光镜下观察胰腺组织病理变化,采用蛋白质印迹法测定胰腺组织中核因子-κB(NF-κB)P65蛋白的水平,采用免疫组织化学和逆转录聚合酶链反应法分别检测胰腺组织中肿瘤坏死因子α(TNF-α)和细胞间黏附因子-1(ICAM-1)的表达情况。结果与假手术组比较,IR组再灌注后相同时点的组织病理损伤明显较重,血清淀粉酶含量、胰腺组织中P65蛋白水平以及TNF-α和ICAM-I水平均明显上升;与IR组比较,MG132组相同时点的组织损伤减轻,血清淀粉酶含量、胰腺组织中P65蛋白水平及TNFa和ICAM-1水平均明显降低。结论MG132能够减轻大鼠移植胰腺的IR损伤,其机制可能是通过抑制NF-κB的活化,从而下调TN-α和ICAM-1的表达。 Objective To investigate the protective effects of Proteasome inhibitor MG132 on ischemi-reperfusion injury (IRI) after pancreas transplantation in rats, and the possible mechanism. Method Fifteen normal SD rats were allocated into the sham operation group. In the allogenie male SD rats, the model of pancreas transplantation was established. The recipients were divided into another two groups (n = 15 each) at random: IRI group and the MG132 pretreatment group. Serum amylase level was determined at 1, 3 and 6 h after the operation. Pancreas samples were harvested at the same time for pathological study by light microscopy. The expression of NF-κB P65 protein in the pancreas was detected by using Western blotting. The expression of TNF-α and ICAM-1 in the pancreas was detected by using immunohistochemistry and RT-PCR. Result Tissue damage on IRI group was more severe than in sham operation group. The level of serum amylase, and the expression of P65, TNF-α and ICAM-1 were higher in IRI group than those in sham operation group (P〈0. 05). Tissue damage in MG132 pretreatment group was milder than in IR1 group. The level of serum amylase, and the expression of P65, TNF-α and ICAM-1 were lower in MG132 pretreatment group than in IRI group (P〈0. 05). Conclusion MG132 pretreatment can alleviate the pancreas IRI after pancreas transplantation, probably by inhibiting the activation of NF-κB, and the inhibition effect can down-regulate the expression of TNF-α and ICAM-1.
出处 《中华器官移植杂志》 CAS CSCD 北大核心 2013年第6期358-361,共4页 Chinese Journal of Organ Transplantation
基金 国家自然科学基金(30871295,81272265) 重庆市科委科技攻关项目(2012gg-yyis10041)
关键词 大鼠 胰腺移植 再灌注损伤 蛋白酶体抑制剂 核因子ΚB Rats Pancreas transplantation Reperfusion injury Proteasome inhibitor NF-κB
  • 相关文献

参考文献3

二级参考文献12

  • 1Clavien PA, Harvey PR, Strasberg SM. Preservation and reperfusion injurics in liver allografts. An overview and synt hesis of current studies [ J ]. Transplantation, 1992,53 ( 5 ) :957 - 978.
  • 2Kataoka M, Shimizu H, Mitsuhashi N, et al. Effect of cold-ischemia time on C-X-C chemokine expression and neutrophil accumulation in the graft liver after orthotopic liver transplantation in rats[ J]. Transplantation ,2002,73 ( 11) :1730 - 1735.
  • 3Serracino-Inglott F, Habib NA, Mathie RT. Hepatic ischemia - reperfusion injury[J]. Am J Surg, 2001,181 (2) :160 -166.
  • 4Ricciardi R, Kim RD, McDade TP,et al. NF kappa B expression during cold ischemia correlates with postreperfusion graft function[J]. J Surg Res, 2000,93(1):35-40.
  • 5Yamamoto Y, Kume M, Yamaoka Y. Implications of heat shock proteins dining liver surgery and liver perfusion[ J]. Recent Results Cancer Res, 1998,147 : 157.
  • 6Tanaka Y, Chen C, Maher JM, et al. Kupffer cell-mediated down- regulation of hepatic transporter expression in rat hepatic ischemia-reperfusion [ J ]. Transplantation, 2006,82 (2) :258 - 266.
  • 7Liu-Mares W, Sun Z, Bamlet WR, et al. Analysis of variation in NF-kappaB genes and expression levels of NF-kappaB-regulated molecules[ J]. BMC Proc,2007,1 ( Suppl1) :S126.
  • 8Tanaka Y, Maher JM, Chen C, et al. Hepatic ischemia-reperfusiun induces renal berne oxygenase-1 via NF-E2-related factor 2 n rats and mice [ J ]. Mol Pharmacol, 2007,71 ( 3 ) :817 - 825.
  • 9TYin H, Huang BJ, Yang H, et al. Pretrcatment with soluble ST2 reduces warm hepatic ischemia/reperfusion injury[ J]. Biochem Biophys Res Commun, 2006,351 (4) :940 - 946.
  • 10明长生,宫念樵,陈孝平.The Current State of Pancreas-kidney Transplantation in China:The Indications,Surgical Techniques and Outcome[J].Journal of Huazhong University of Science and Technology(Medical Sciences),2009,29(3):269-272. 被引量:1

共引文献10

同被引文献19

  • 1Kioda K,Domanski L, Pawlik A, et al. The impact ofICAM1 and VCAM1 gene polymorphisms on chronicallograft nephropathy and transplanted kidney function[J]. Transplant Proc, 2013,45 (6) : 2244-2247.
  • 2Kloda K,Domafiski L,Pawlik A, et al. The impact ofICAM1 and VC AMI gene polymorphisms on long-termrenal transplant function and recipient outcomes[ J]. AnnTransplant, 2013,18: 231-237.
  • 3Mannam VK,Lewis RE,Cruse JM. The fate of renalallografts hinges on responses of the microvascularendothelium [ J ] . Exp Mol Pathol, 2013,94 ( 2 ):398-411.
  • 4Cho HK,Kim SY,Seong JK,et al. Hepatitis B virus Xincreases immune cell recruitment by induction ofchemokine SDF-1 [ J ] . FEBS Lett, 2014,588 (5):733-739.
  • 5Flameng W, De Visscher G, Mesure L,et al. Coatingwith fibronectin and stromal cell-derived factor-la ofdecellularized homografts used for right ventricularoutflowtract reconstruction eliminates immune response-relateddegeneration[ J]. J Thorac Cardiovasc Surg, 2014, 147(4); 1398-1404.
  • 6Paller MS, Hoidal JR, Ferris TF. Oxygen free radicals in isehemic acute renal failure in the rat[ J]. J Clin Invest, 1984, 74 (4): 1156-1164.
  • 7Shayegi N,Meyer C,Lambert K,et al. CXCR4 blockadeand sphingosine-1 -phosphate activation facilitateengraftment of haematopoietic stem and progenitor cells ina non-myeloablative transplant model [ J ] . Br JHaematol, 2014,164 (3) : 409-413.
  • 8Yang D,Sun S, Wang Z, et al. Stromal cell-derivedfactor-1 receptor CXCR4-overexpressing bone marrowmesenchymal stem cells accelerate wound healing bymigrating into skin injury areas [ J ] . Cell Reprogram,2013,15 (3) : 206-215.
  • 9Xu X,Zhu F,Zhang M,et al. Stromal cell-derivedfactor-1 enhances wound healing through recruiting bonemarrow-derived mesenchymalstem cells to the wound areaand promoting neovascularization [ J ] . Cells TissuesOrgans, 2013,197 (2) : 103-113.
  • 10Xargay-Torrent S, Lopez-Guerra M, Montraveta A, et al.Sorafenib inhibits cell migration and stroma-mediatedbortezomib resistance by interfering B-cell receptorsignaling and protein translation in mantle cell lymphoma.

引证文献1

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部